Substance extracted from giant knotweed for diabetes mellitus and complication thereof
A technology for diabetes and complications, which is applied in the field of substances for the treatment of diabetes and complications, and can solve the problems of unclear hypoglycemic active sites and active ingredients
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[0011] According to the foregoing technical solutions, the present invention is implemented in the following specific manners in implementation.
[0012] The rhizomes and roots of Polygonum cuspidatum are removed from the soil, washed, and crushed into coarse powder (50-80 mesh). Take 1000 grams of coarse powder, add 6000ml of 70% ethanol and soak for 30 minutes, reflux and extract for 2 hours, filter, Obtain the medicinal solution for use, add 6000ml of ethanol with a mass concentration of 70% to the medicinal residue, extract at reflux for 2h, filter, combine the filtrate twice, recover the ethanol under reduced pressure, and concentrate to 50% of the volume, then add the pretreatment and regenerated macropores In the adsorption resin column (the pretreatment and regeneration of the macroporous adsorption resin column are the same as before), elute with distilled water until the eluent is colorless, and then elute with ethanol with a mass concentration of 70%, and collect the el...
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