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Rebaudioside A and extraction method thereof

一种莱鲍迪甙、提取方法的技术，应用在莱鲍迪甙A及其提取领域，能够解决不能得到莱鲍迪甙A、不能分离开等问题，达到成本低、处理量大的效果

Inactive Publication Date: 2008-06-18
CHENGDU WAGOTT RUITIAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor's repeated experiments have shown that such a simple treatment step cannot separate rebaudioside A from other components that are very similar in structure, so in practice, the claimed purity of 99% rebaudioside A cannot be obtained through this patented method Diglycoside A

Method used

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  • Rebaudioside A and extraction method thereof
  • Rebaudioside A and extraction method thereof
  • Rebaudioside A and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0050] Embodiment 2 The impact of sample silica gel ratio on refining 70% rebaudioside A

[0051] According to the process of Example 1, the stevioside containing 70% rebaudioside A was refined, and the results are shown in Table 2.

[0052] Table 2 Effect of the ratio of stevioside silica gel on refining 70% rebaudioside A

[0053]

[0054] The above experimental results show that rebaudioside A with a purity ≥ 99% can be purified from stevioside through the refining process of the present invention. And when the weight ratio of stevioside to silica gel is 1:4-1:5, the purification effect is the best.

[0055] The present invention is not limited to the specific content of rebaudioside A and the specific ratio of stevioside and silica gel in the examples.

Embodiment 3

[0056] Example 3 The influence of the proportion of chromatographic eluent on refining 20% ​​rebaudioside A

[0057] Carry out silica gel column chromatography to 4 parts of each 50.0g stevioside (containing 20% ​​rebaudioside A) according to the sample silica gel ratio of 1: 4 (m / m), and with ethyl acetate: ethanol / water (10 / 1, v / v)=20:1~20:4 (v / v) mixed solvent for elution, and each fraction was collected separately. The rebaudioside A fractions were determined by thin-layer chromatography and then combined and concentrated to obtain rebaudioside A as a solid. Put the solid in a 250ml Erlenmeyer flask, add 5% (v / v) mixed solvent of water and ethanol at a ratio of 1:4 (m / v), heat and stir at 60°C for 4 hours, freeze and stir at -20°C for crystallization After 24 hours, the crystals were collected by filtration. The crystals were dried at 80°C to constant weight, weighed, and samples were taken for purity identification by HPLC. The experimental results are shown in Table 3...

Embodiment 4

[0062] Embodiment 4 The influence of chromatographic eluent ratio on refining 70% rebaudioside A

[0063] According to the process of Example 1, the stevioside containing 70% rebaudioside A was refined, and the results are shown in Table 4.

[0064] The impact of table 4 chromatographic eluent ratio on refining 70% rebaudioside A

[0065]

[0066] The above experimental results show that rebaudioside A with a purity ≥ 99% can be purified from stevioside through the refining process of the present invention. And the best purification effect is obtained when the volume ratio of ethyl acetate:ethanol / water (10 / 1, v / v) is 20:2-20:3.

[0067] The present invention is not limited to the specific content of rebaudioside A and the specific ratio of the chromatographic eluent in the examples.

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Abstract

The invention provides rebaudioside A with the purity of more than 99 percent. The invention also provides an extraction method of the rebaudioside A. The rebaudioside A with high purity provided by the invention can be purified to rebaudioside A with the purity of more than 99 percent by the process. The yield of rebaudioside A is higher than 85 percent. Rebauidoside A from the invention has no bad aftertaste on the mouthfeel, and can be used as the sweetener. The invention has the advantages of large production amount, low cost and being suitable for the industrial production.

Description

technical field [0001] The invention relates to rebaudioside A and an extraction method thereof, belonging to the field of food chemical industry. Background technique [0002] Stevioside (Total steviosides) is a high-sweetness, low-calorie natural health-care sweetener extracted from the leaves and stems of Stevia rebaudiana Bertohi. It mainly contains eight different sweet components: stevioside (Stevioside); Rebaudioside (Rebaudioside) A, B, C, D, E and dulcoside (Dulcoside) A, B. Among them, stevioside accounts for about 66% of the total glycosides, rebaudioside A accounts for about 22% of the total glycosides, rebaudioside C accounts for about 9% of the total glycosides, and dulcoside A accounts for about the total glycosides. 2%, and the other ingredients are very small. [0003] Common steviosides have some defects, and most of their sweet components have a strong aftertaste, which restricts the wide application of steviosides. Rebaudioside A is the sweetest ingred...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/20C07H1/06A23L1/236A23L27/30
CPCA23L1/3002A23L33/105
Inventor 阳明福华君秦岭许庆
Owner CHENGDU WAGOTT RUITIAN TECH CO LTD
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