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Processing method before imbedding biological specimen

A technology of biological specimens and processing methods, applied in the field of biological specimen embedding, can solve the problems of high cost, specimen deformation, complicated operation, etc., and achieve the effects of cost reduction, good embedding effect and simple operation

Inactive Publication Date: 2008-05-21
CHONGQING NORMAL UNIVERSITY
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Problems solved by technology

[0002] At present, the treatment method of biological specimens before embedding generally adopts direct dipping after drying specimens or embedding without any treatment. When this method is used for processing, the specimen with high water content is easily deformed during the drying process, and the original shape of the specimen cannot be fully expressed.
The Chinese patent with application number 200510020434.9 discloses a method of plasticizing specimens with polyethylene glycol. This method uses different concentration gradients of polyethylene glycol for treatment. Although it can effectively remove water, the cost is high and the operation is relatively complicated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] Embedding of Flammulina velutipes specimens

[0012] (1) Preparation of treatment solution: take 40 milliliters of formaldehyde with a concentration of 37-40%, add 60 grams of urea, heat to 40-50°C and stir until the urea is completely dissolved, set aside;

[0013] (2) directly put the collected Flammulina velutipes into the treatment solution and soak for 1 to 3 days;

[0014] (3) Take out the soaked Flammulina velutipes and put it into a porcelain plate with absorbent paper, absorb the excess treatment liquid on the surface of the specimen with absorbent paper, transfer it to a 30-40°C incubator and place it for 1-4 hours before use;

[0015] (4) The transparent urea-formaldehyde resin is completely fused with the biological specimen treated in step (3) for specimen embedding.

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Abstract

The invention relates to a processing method of biological specimens before embedment. The method is that the biological specimens is dipped into a processing solution prepared with formaldehyde withthe concentration of 37 to 40 percent per 37.5 to 42.5ml and 57.5 to 62.5g of carbamide for 1 to 3 days and then the specimens are transferred to place in an incubator of 30 to 40 DEG C for 1 to 4 hours and then the specimens are embedded with transparent urea-formaldehyde resin. The processing method has the advantages of effectively moisture removing, lower cost, simple operation, better embedment effect and anti-mildew, anti-pest and color preserving, and is especially applicable to the embedment of biological specimens with more water content, such as Flammulina, Craspedacusta, earthworms,small mollusks and fish, etc.

Description

technical field [0001] The invention relates to an embedding method of a biological sample, in particular to a treatment method of the biological sample before embedding. Background technique [0002] At present, the treatment method of biological specimens before embedding generally adopts direct dipping after drying specimens or embedding without any treatment. When this method is processed, the specimen with high water content is easily deformed during the drying process, and the original shape of the specimen cannot be fully expressed. The Chinese patent with application number 200510020434.9 discloses a method of plasticizing specimens with polyethylene glycol. This method uses different concentration gradients of polyethylene glycol for treatment. Although it can effectively remove water, the cost is high and the operation is relatively complicated. . Contents of the invention [0003] Aiming at the deficiencies of the prior art, the present invention provides a tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G09B23/00
Inventor 唐安科唐发辉赵元莙
Owner CHONGQING NORMAL UNIVERSITY
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