Method for steam-exploded straw hydrogen production by controlling temperature of fermentation process

A technology of steam-exploding straw and fermentation process, which is applied in the field of fermentation technology, which can solve the problems of inconsistency between enzymatic hydrolysis and fermentation temperature, variable temperature stimulation, etc., and achieve the effects of improving utilization rate, promoting cellulose enzymatic hydrolysis, and increasing product yield

Inactive Publication Date: 2008-05-14
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of inconsistency between enzymatic hydrolysis and fermentation temperature in the hydrogen production process of synchronous saccharification and fermentation of steam-exploded straw, and to provide a temperature-changing stimulation during the fermentation process to meet the needs of cellulose degradation and microbial fermentation at the same time, and improve fiber production. A new method for enzymatic hydrolysis rate and hydrogen production rate

Method used

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  • Method for steam-exploded straw hydrogen production by controlling temperature of fermentation process

Examples

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Embodiment 1

[0028] Embodiment 1. Use the method provided by the invention to use steam-exploded corn stalks as raw material to carry out variable temperature fermentation to prepare hydrogen, and the specific steps are as follows:

[0029] 1. Preparation of steam-exploded straw material

[0030] Crush the straw to a length of 3 to 4 cm, put it into the steam explosion tank, and pass water steam into it after airtight to make the tank pressure rise to 1.5MPa rapidly, maintain this pressure for 10 minutes, then release the pressure quickly to obtain the steam explosion straw material ;

[0031] 2. Preparation of Clostridium butyricum seed solution

[0032] Inoculate Clostridium butyricum (purchased from the General Microorganism Collection Center of China Microorganism Collection Management Committee, its preservation number is CGMCC AS1.209) in Clostridium butyricum culture medium, anaerobically cultured at 35°C to logarithmic growth phase, and then obtained Clostridium butyricum seed so...

Embodiment 2

[0041] Embodiment 2. ferment with steam-exploded sorghum stalk as raw material with the method provided by the invention, and concrete steps are as follows:

[0042] Steps 1 and 2 are the same as in Example 1.

[0043] 3. Add urea 0.15g / g-substrate, KH 2 PO 4 0.03g / g-substrate, MgSO 4 0.015g / g-substrate and water, the ratio of solid to liquid mass parts is 1:3, and the steam-exploded straw medium is obtained after sterilization; the substrate refers to the raw material of steam-exploded straw;

[0044] 4. Mix the seed solution of C.butyricum AS1.209 in the logarithmic growth phase with the medium evenly, inoculate 600 million cells / g-substrate, and add sterile distilled water to the medium to make the final solid The liquid mass ratio is 1:5, and nitrogen gas is quickly introduced to deoxygenate, and the fermentation is started at 35°C. 10 hours after the start of fermentation, the temperature of the reactor was rapidly raised to 45° C., maintained for 1 hour, then rapidl...

Embodiment 3

[0046] Embodiment 3. use method provided by the invention to ferment with steam-exploded wheat straw as raw material, and concrete steps are as follows:

[0047] Steps 1 and 2 are the same as in Example 1.

[0048] 3. Add urea 0.18g / g-substrate, KH to the raw material of steam-exploded straw 2 PO 4 0.03g / g-substrate, MgSO 4 0.015g / g-substrate and water, the ratio of solid to liquid mass parts is 1:3, and the steam-exploded straw medium is obtained after sterilization; the substrate refers to the raw material of steam-exploded straw;

[0049] 4. Mix the seed solution of C.butyricum AS1.209 in the logarithmic growth phase with the medium evenly, inoculate 800 million cells / g-substrate, and add sterile distilled water to the medium to make the final solid The liquid mass ratio is 1:7, and nitrogen gas is quickly introduced to deoxygenate, and the fermentation is started at 35°C. After 8 hours of fermentation, the temperature of the reactor was rapidly raised to 43° C., and a...

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Abstract

The invention discloses a hydrogen production method by using steam-exploded straw fermentation and adjusting the temperature of the fermentation process. The method comprises the steps that: the steam-exploded straw is added with carbamide, KH2PO4, MgSO4 and water to obtain a steam-exploded straw culture medium; the butyric acid clostridium is inoculated in a seed culture medium and an anaerobic culture at 35 DEG C is conducted to logarithmic phase so as to obtain seed liquid; the steam-exploded straw is inoculated with the seed liquid and is deoxidized through introducing nitrogen; the obtained material is fermented for 6 to 12 hours under the temperature of 35 DEG C, then fast heated to 40 to 46 DEG C and maintained for 1 to 3 hours; the temperature of the obtained material is fast lowered to 35 DEG C to continuously ferment under constant temperature; the ferment lasts 22 to 36 hours, and then the temperature is heated once again to 40 to 46 DEG C and maintained for 1 to 3 hours; the temperature of the obtained material is lowered to 35 DEG C to ferment under constant temperature till no gas produces; the generated gas is separated to make pure hydrogen. By using the method, the temperatures are enhanced a plurality of times for a short time during fermentation process, which can promote substrate cellulose to be degraded; since the temperature enhancing time is shorter, the microorganism activity has a little effect and the hydrogen yield is greatly enhanced.

Description

technical field [0001] The invention belongs to the technical field of fermentation technology, and mainly relates to a method for regulating hydrogen production by fermentation of steam-exploded straw by using temperature changes in the fermentation process. Background technique [0002] With the development of human society, the demand for energy is increasing. However, the reserves of fossil fuels on the earth are limited. Therefore, new renewable energy must be developed to meet the needs of human production and life. Hydrogen is a clean and efficient energy source and a good substitute for fossil fuels. Conventional hydrogen production methods, such as electrolysis of water and pyrolysis of fossil raw materials [Adnan M.Int J Hydrogen Energy, 2001: 26: 29-27; Ayhan D. Energy Sources, 2002: 24: 59-68.], exist The disadvantages of high energy consumption, complex product components, and high separation costs cannot fundamentally solve energy and environmental problems. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/145
Inventor 陈洪章李冬敏
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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