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Affinity foam fractionation for collection and purification of materials

A foam, substance technology for the purification and/or concentration of compounds that solves the problems of underdeveloped foam fractionation, lack of distribution activity, and lack of understanding of the method

Inactive Publication Date: 2008-04-23
THE UNIVERSITY OF AKRON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] [0007] Despite its promising potential, foam fractionation has not been greatly developed due to a lack of understanding of the method
In addition, the inventors have found that the product of interest does not have the highest partition activity of all substances present in the culture broth containing the product
Based on this, simple foam fractionation processes / methods mentioned in the literature cannot give acceptable results

Method used

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  • Affinity foam fractionation for collection and purification of materials
  • Affinity foam fractionation for collection and purification of materials
  • Affinity foam fractionation for collection and purification of materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 - Affinity foam fractionation with addition of cellulose hydrolyzate

[0076] [0051] The experimental results, summarized in Tables 1 and 2 appended hereto, show the typical influence of three factors on foaming behavior. The factors are: (1) the presence or absence of cells in the foaming medium; (2) the presence of different growth stages of the cells; and (3) the addition of hydrolyzate. Cells used in the cell-containing system were pre-grown in glucose-based medium (containing 10 g / L glucose). To study the effect of different growth phases, cells were harvested in late exponential growth (day 3 of batch culture) or stationary growth phase (day 15). The culture supernatant, which was used in all systems as the basal medium for the production of cellulase, was prepared by fermentation with the aid of a hydrolyzate-based medium. The broth was harvested on day fifteen and centrifuged to remove cells. Using the same basal culture supernatant helps ensure tha...

Embodiment 2

[0092] Example 2 - Affinity foam fractionation with addition of CMC:

[0093] [0060] CMCs are modified, water-soluble, long-chain cellulose analogs. The potential application of CMCs for affinity foam fractionation of cellulases is discussed below. Experiments were performed on cell-free broth supernatant collected from hydrolyzate-based fermentations. To obtain a higher FPU (approximately 0.7) than the FPU of earlier batch cultures (approximately 0.3-0.4 FPU), the fermentation was supplemented with a continuous lactose-based feed after the steady state phase was reached. Three systems were compared: (a) culture supernatant (control); (b) supernatant added with 5% 5-g / L CMC solution; and (c) added 5% hardwood CH ( Containing the supernatant of reducing sugar of 16g / L). The ERs of FPU, extracellular proteins and reducing sugars are shown in FIG. 5 . CMC solutions were found to perform as well, if not better, than CH in terms of cellulase enrichment.

[0094] [0061] Sever...

Embodiment 3

[0095] Example 3 - Affinity foam fractionation with addition of xylan hydrolyzate:

[0096] [0062] Table 3 presents the results of experiments comparing the effect of xylan hydrolyzate (XH) and cellulose (hardwood) hydrolyzate (CH) in cellulase affinity foam fractionation. Cell-free broth supernatants were collected from lactose-based fermentations as described in the Materials and Methods section. Lactose based broths, although having a much higher FPU (approximately 0.9), did not prove to have very good foaming properties. Poor bubbling was associated with much lower concentrations of extracellular proteins, confirming earlier observations that cellulases do not elicit efficient bubbling compared to certain other proteins present in the culture broth, and proceed through foam fractionation. The selective separation of cellulose requires the affinity bubbling developed in this work.

[0097] [0063] XH has a stronger foaming ability than CH, as shown in Table 3 that XH obt...

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Abstract

The present invention generally relates to methods for purifying and / or concentrating compounds from or in solutions and / or mixtures. In one embodiment, the present invention relates to a method for purifying and / or concentrating a compound from a solution or mixture. In another embodiment, the present invention relates to a method for purifying / concentrating a compound from a solution or mixture that utilizes, in whole or part, foam purification and / or concentration. In still another embodiment, the present invention can be used to separate, concentrate and / or purify any material, including biological products and / or biomaterials, that can be selectively bound to a binding agent, thereby yielding a complex that will readily partition onto bubble surfaces in a foam.

Description

field of invention [0001] [0001] The present invention generally relates to methods of purifying and / or concentrating compounds from or in solutions and / or mixtures. In one embodiment, the invention relates to methods of purifying and / or concentrating compounds from solutions or mixtures. In another embodiment, the present invention relates to a method of purifying and / or concentrating a compound from a solution or mixture, utilizing foam purification and / or concentration in whole or in part. In yet another embodiment, the present invention can be used to isolate, concentrate and / or purify any substance, including biological products and / or biological materials, which can selectively bind to a binding agent, thereby producing a complex, said The complex will readily distribute to the bubble surface of the foam. Background of the invention [0002] [0002] Biological products are often present in dilute mixtures or solutions during or at the end of production. Therefore, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C1/10B03D1/00B03D1/14C02F1/24C08B30/04
CPCB03D1/008B03D1/016B03D2203/001B03D1/028B03D2203/003B03D2203/005B03D2201/007B03D2201/02
Inventor L-K·居Q·张
Owner THE UNIVERSITY OF AKRON
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