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Hypotensive agent produced by cultivation of lactic acid bacterium

A technology of lactic acid bacteria culture and antihypertensive agent, which is applied in the direction of bacteria, dairy products, cardiovascular system diseases, etc., and can solve the problems of high blood pressure without knowing whether it is effective

Inactive Publication Date: 2008-04-09
DAINIPPON INK & CHEM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although it is known to maintain a mixture containing spirulina or chlorella and lactic acid bacteria in the presence of water and culture the lactic acid bacteria, and to use the resulting culture as a health food, the fact that it is effective for hypertension is still unknown. totally unaware

Method used

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  • Hypotensive agent produced by cultivation of lactic acid bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (Example 1) Preparation (1) of lactic acid bacteria culture using spirulina and measurement of the number of lactic acid bacteria

[0076]25kg of galacto-oligosaccharides and 825kg of tap water are loaded into a culture tank with a capacity of 2500L. After heat sterilization, 100kg of spirulina bulk material is added, and 50kg of seed culture solution of lactic acid bacteria Lactobacillus brevis is inoculated. Lactic acid bacteria were cultured at 37°C for 72 hours under agitation with an aerated propeller. The culture solution with the culture time of 0, 9, 12, 15, 18, 24, 48 and 72 hours was sampled to measure the number of lactic acid bacteria.

[0077] In addition, the measurement of the number of lactic acid bacteria was performed in the following procedure. Suspend 1.0 ml of lactic acid bacteria culture solution in 19 ml of phosphate-buffered saline to prepare a suspension. The suspension was further diluted 10-fold, 10-fold with phosphate-buffered saline 2 tim...

Embodiment 2

[0088] (Example 2) Preparation of lactic acid bacteria culture using spirulina (2)

[0089] In this example, lactic acid bacteria were cultivated in the same manner as in Example 1, except that the pH was maintained at 5.0 with a 50% (w / w) lactic acid solution and the culture temperature was maintained at 30°C. The culture solution with the culture time of 0, 9, 12, 15, 18, 21 and 24 hours was sampled, and the amount of GABA (mg / 100g) was measured.

Embodiment 3

[0090] (Example 3) Preparation of lactic acid bacteria culture using spirulina (3)

[0091] In this example, except that xylooligosaccharide was used instead of galactooligosaccharide, the same operation was performed as in Example 2, and the amount of GABA (mg / 100g) was measured.

[0092] The comparison of the GABA amount (mg / 100g) of Example 1 and Example 2 up to the culture time of 24 hours is shown in FIG. 1, and the result of Example 2 and Example 3 is shown in Table 2.

[0093] Through Examples 1-3, the appropriate pH and culture temperature are selected, so that more GABA can be produced in a short time.

[0094] Table 2

[0095] GABA content (mg / 100g)

[0096] Training time

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PUM

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Abstract

A hypotensive agent comprising, as the active ingredient, a culture of a lactic acid bacterium which is produced by cultivating the lactic acid bacterium in a culture medium containing a microalga and the lactic acid bacterium.

Description

technical field [0001] The present invention relates to a hypotensive agent, which uses the culture obtained by cultivating lactic acid bacteria in a specific culture solution as an active ingredient. Background technique [0002] In the past, foods containing spirulina or chlorella have been used as foods that can easily ingest nutrients that are often lacking in daily diets because they contain rich nutrients unique to or inherent in green and yellow vegetables. . Recently, it has been proposed to cultivate lactic acid bacteria in spirulina or chlorella to improve the peculiar smell and flavor of spirulina and chlorella (refer to Patent Documents 1 and 2). [0003] In addition, specific health foods are said to be effective for hypertension, and it is known that the method of inoculating milk with lactic acid bacteria (Patent Document 3), the method of inoculating dairy products with enzymes, and then inoculating lactic acid bacteria (Patent Document 4), or A health food...

Claims

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Application Information

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IPC IPC(8): A61K36/02A61K35/74A61K36/05A61P9/12A23C9/13A61K31/197A61K35/744A61K35/747A61K36/03
CPCC12N1/20A61K36/05A61K2035/11A61K35/744A61K36/02A61P9/12A61K2300/00A61K35/74
Inventor 佐渡山惠一宫城健稻福桂一郎榊原正树平桥智裕吉川典孝
Owner DAINIPPON INK & CHEM INC
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