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Method of providing readily available cellular material derived from peripheral blood, and a composition thereof

A technology of peripheral blood stem cells and compositions, which is applied in the field of providing easily obtained cell materials and compositions derived from peripheral blood, which can solve the problems of difficult positioning, few pluripotent cell functions, and small quantities

Inactive Publication Date: 2008-04-09
REGENETECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although adult stem cells can be found in many mature tissues, they are found in smaller numbers and are more difficult to locate
In addition, stem cells found in a tissue may be specialized to that tissue, rarely functioning as true pluripotent cells

Method used

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  • Method of providing readily available cellular material derived from peripheral blood, and a composition thereof
  • Method of providing readily available cellular material derived from peripheral blood, and a composition thereof
  • Method of providing readily available cellular material derived from peripheral blood, and a composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1 - Practical TVEMF amplification in a TVEMF bioreactor

[0101] Peripheral blood was collected and peripheral blood cells expanded as shown in Table 1 below.

[0102] A) Harvesting and maintaining cells

[0103] Human peripheral blood (approximately 0.75 × 10 6 cells / ml) and suspended in Iscove's modified Dulbecco's medium

[0104] Example 1 - Practical TVEMF amplification in a TVEMF bioreactor

[0105] Peripheral blood was collected and peripheral blood cells expanded as shown in Table 1 below.

[0106] A) Harvesting and maintaining cells

[0107] Human peripheral blood (75 ml; approximately 0.75 x 10 6 cells / ml) and suspended in Iscove's Modified Dulbecco's Medium (IMDM) (GIBCO, Grand Island, NY), in which 20% of 5% human albumin (HA), 100ng / ml recombinant human G-CSF (Amgen Inc ., Thousand Oaks, CA), and 100 ng / ml recombinant human stem cell factor (SCF) (Amgen). The peripheral blood mixture was placed in a TVEMF bioreactor as shown in Figures 2 and 3...

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Abstract

The present invention relates to the TVEMF amplification of the optimized CD34+ / CD38 cell of the stem cell in the periphery and external blood of the mammalian, also relates to the composition from the TVEMF amplification cell, and a method for treating the disease or restoring the tissue with the composition.

Description

technical field [0001] The present invention relates to adult stem cells from peripheral blood prepared in TVEMF-bioreactors, as well as methods of this preparation, compositions thereof and methods of using said cells or compositions for the treatment of mammals. technical background [0002] Mammalian, especially human, tissue regeneration has long been a medical ideal. To date, human tissue repair has largely been achieved by transplanting similar tissue from a donor. Basically starting with the kidney transplant from one of the twins Herrick to the other, and later the world famous heart transplant from Denise Darval to Louis Washkansky performed by South African doctor Christian Barnard on December 3, 1967, tissue transplants became the standard for A widely accepted method of prolonging life in terminally ill patients. [0003] Human tissue transplantation has encountered major problems since its initial application, mainly due to tissue rejection by the body's natur...

Claims

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Application Information

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IPC IPC(8): A01N1/02A01N37/18C12N5/06A61K35/12C12M1/00A61K35/28C12N5/0789
CPCY02A50/30
Inventor 唐尼·拉德大卫·A·沃尔夫
Owner REGENETECH INC
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