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Bacterium agent for renovation of organic pollution aquifer, producing and using method of the same

A technology for inoculants and water bodies, applied in the field of microorganisms, can solve the problems of low cost, less nitrobenzene polluted water bodies, etc., and achieve the effects of low cost, strong reproductive ability and adaptability, and simple use methods.

Inactive Publication Date: 2011-04-13
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

This is due to the fact that bioremediation technology is less costly than physical and chemical restoration technology, has no secondary pollution, and microorganisms have strong variability and adaptability. Therefore, the use of biological methods to remediate nitrobenzene-contaminated water bodies is the most ideal method, but about There are still few reports on the remediation of water bodies polluted by nitrobenzene in the environment

Method used

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Experimental program
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Effect test

Embodiment 1

[0020] The bacterial agent for repairing organically polluted water bodies is composed of facultative anaerobic microorganisms, and the facultative anaerobic microorganisms can be: Pseudomonas stutzeri, Alealigenesfaecalis, Bacillus megaterium ), Enterobacter cloacae (the above strains can be purchased from the market), the ratio of parts by weight is 1:1.5:2.5:5, and the balance is the culture solution containing nitrobenzene.

[0021] The preparation method of bacterial agent: the facultative anaerobic microorganism of 10% by weight is inoculated in the anaerobic bottle that 400ml sterilized culture solution is housed, and the initial concentration of nitrobenzene reaches 30mg / L in the culture solution, seals, and places in 30 Cultivate in an incubator at ℃ for 5 days, then take out 1 / 3 of the culture solution, add the same amount of culture solution and add nitrobenzene solution at the same time, so that the content of nitrobenzene in the medium reaches the initial concentra...

Embodiment 2

[0028] The bacterial agent for repairing organically polluted water is composed of facultative anaerobic microorganisms, which can be: Pseudomonas stutzeri, Alealigenesfaecalis, Bacillus megaterium ), Enterobacter cloacae (Enterobacter cloacae), the ratio of parts by weight is 1:1:2:6, and the balance is the culture solution containing nitrobenzene.

[0029] The preparation method of bacterial agent: the facultative anaerobic microorganism that is 5% by weight is inoculated in the anaerobic bottle that the sterilized culture fluid that contains 5ml nitrobenzene of 400ml is housed, and the initial concentration of nitrobenzene reaches 80mg in the culture medium / L, sealed, and placed in a 30°C incubator for 4 days, then take out 1 / 5 of the culture solution, add the same amount of culture solution and add nitrobenzene solution at the same time, so that the content of nitrobenzene in the medium reaches the initial concentration , and continue to cultivate in the incubator for 4 d...

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Abstract

The invention relates to the microbial field, in particular to a microorganism agent for recovering the organic polluted water and preparation process and using method. The microorganism agent consists of facultative anaerobics including Pseudomonas, Alcaligenes, Bacillus and Enterbacter with the weight ratio of 1:1-3:1-4:2-7, and the residual is the culture liquid containing nitrobenzene; the degradation rate can reach between 97.7 percent and 99.6 percent when the invention is implemented in the polluted water containing nitrobenzene. The microorganism agent of the invention is made by thatthe nitryl aromatic hydrocarbon compound and the nutrition compound with relatively high concentration are acclimatized and sorted, and has strong growing and reproductive ability as well as adaptability; in addition, the invention can assure a higher biomass in the environment at low cost, and does not cause the secondary pollution, and has the effect of continuously strengthening the purification of the water, thereby having good social, economic and ecological benefits.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent for repairing organically polluted water bodies and its preparation and use methods. Background technique [0002] The pollution of natural water bodies (including sediment) with cities as the center and rivers as the link caused by the rapid development of industry and agriculture is an environmental problem that the world expects to solve. A large amount of industrial waste and accidents produced by my country's chemical and petrochemical industries can easily cause pollution of drinking water sources along rivers, lakes and seas. The nitrobenzene pollution accident in Jihua's double benzene workshop seriously polluted the water body of Songhua River and caused huge international impact. A chemical group in Liaoning discharges more than 7 million tons of waste water containing nitrobenzene compounds every year, and its pollution level far exceeds the pollution c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12R1/07C12R1/05C12N1/20
Inventor 李培军许华夏张春桂张海荣尹炜鞠京丽
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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