Escherichia coli biochemistry identification method
A biochemical identification technology for Escherichia coli, which is applied in the field of microbiology, can solve problems such as complicated procedures, limited work for identification of Escherichia coli, and various projects
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[0013] In the national 863 project undertaken by the applicant. 343 strains of fecal coliform were obtained by collecting human, pig, cow, and chicken feces, and 290 strains of Escherichia coli were obtained by using the improved identification method, which saved money and time.
[0014] Table 3. Identification results of Escherichia coli using the improved identification method
[0015] sample source
[0016] In order to verify the accuracy of the improved identification method, the inventors verified it with the most authoritative identification method at present—the 16SrDNA gene sequencing method. From the 290 strains of Escherichia coli that were identified, 8 strains of Escherichia coli that had been biochemically identified were randomly selected for 16SrDNA gene sequencing and identification. The similarity rate is more than 99%, and the phylogenetic tree is shown in the attached figure, which fully verifies the feasibility of the identification method.
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