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Process for producing feeding micro-ecological preparation by solid fermentation of brewery mash with various bacterium and fermentation culture medium thereof

A micro-ecological preparation and solid-state fermentation technology, which is applied in the field of feed industry and animal husbandry and breeding industry, can solve the problems such as non-multi-bacterial solid-state fermentation of brewer's grains, etc., and achieve the effects of easy control of fermentation process, low price and simple production facilities.

Active Publication Date: 2008-03-12
CHANGSHA LVYE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on the production of feed protein using brewer's grains at home and abroad, but so far there are no relevant reports on the production of microecological preparations for multi-bacterial solid-state fermentation of brewer's grains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preservation status of the three bacteria:

[0020] Bacteria D, E, and F have been preserved in the China Center for Type Culture Collection, and the preservation date is March 16, 2007.

[0021] CCTCC deposit number

Strain number

strain name

CCTCC M 207096

CCTCC M 207097

CCTCC M 207098

Strain D

strain E

Strain F

Lactobacillus delbrueckii

Bacillus subtilis

Strange Yeast (Saccharomyces paradozus)

[0022] Lactobacillus delbrueckii belongs to Lactobacillus, Bacillus subtilis belongs to Bacillus; Saccharomyces mirabilis belongs to yeast.

[0023] 2. Morphological, physiological, biochemical and metabolic characteristics of bacteria D, E, and F:

[0024] Morphological, physiological, biochemical and metabolic characteristics of strain D

[0025] Test items

result

Test items

result

Test items

result

s

cell shape

Cell diameter > 1.0μm

en...

Embodiment 2

[0036] Others are with embodiment 1. Take a ring of bacterial lawn from the inclined surface of the test tube to inoculate D, E, and F bacteria into 50ml MRS liquid medium, 50ml broth liquid medium, and 50ml potato dextrose liquid medium, and culture at a constant temperature of 37°C for 21 hours. The suspension was mixed in a volume ratio of 2:1:2 (D bacteria: E bacteria: F bacteria), and added to the sterilized culture medium with brewer's grains as the main raw material in a ratio of 12% (V / W). Mix evenly, then ferment at 32°C for 42 hours, and then freeze-dry.

[0037] The physical and chemical characteristics and biological properties of the final product: yellowish brown, powdery; water content ≤ 10%; the total number of beneficial bacteria containing 9 × 10 8 CFU / g~1×10 10 CFU / g, of which D bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, E bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, F bacteria content is 1×10 8 CFU / g~1×10 10 CFU / g.

Embodiment 3

[0039] Others are with embodiment 1. Take a ring of bacterial lawn from the inclined surface of the test tube to inoculate D, E, and F bacteria into 50ml MRS liquid medium, 50ml broth liquid medium, and 50ml potato dextrose liquid medium, and culture at a constant temperature of 37°C for 24 hours. The suspension was mixed at a volume ratio of 1:1:2 (D bacteria: E bacteria: F bacteria), and added to the sterilized culture medium with brewer's grains as the main raw material in a ratio of 15% (V / W). Mix evenly, then ferment at 34°C for 48 hours, and then freeze-dry.

[0040] The physical and chemical characteristics and biological properties of the final product: yellowish brown, powdery; water content ≤ 10%; total number of beneficial bacteria 9 × 10 8 CFU / g~1×10 10 CFU / g, of which D bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, E bacteria content is 4×10 8 CFU / g~1×10 10 CFU / g, F bacteria content is 1×10 8 CFU / g~1×10 10 CFU / g.

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Abstract

The invention provides a process for producing forage micro ecologic preparations by a multi-bacteria solid fermentation beer draff and a fermentation culture medium for the preparations. Delbrueckii subsp bulgaricus (preservation no. CCTCC M 207096), bacillus subtilis (preservation no. CCTCC M 207097) and saccharomyces paradoxus (preservation no. CCTCC M 207098) are respectively inoculated to corresponding liquid culture media, cultured 18-24 h thermostatically under 37 DEG C; then the suspension fluids are inculcated in certain proportion into a disinfected solid fermentation culture media taking beer draff as main raw materials, fermented for 36-48h under 30-34 DEG C, and frozen dry. In this way the forage micro ecologic preparations are got. The production process in the invention is of low investment, low energy consumption, high yield and free from pollution from waste fluid, and opens a new means for changing waste into a treasure for beer draff.

Description

technical field [0001] The invention relates to the fields of feed industry and animal husbandry, in particular to a production process and a fermentation medium for feed microecological preparations in feed additives. Background technique [0002] Feed microecological preparations are biological preparations or live preparations containing live bacteria that are produced by using known beneficial microorganisms through special processes such as cultivation, fermentation, drying, and processing, and are used for animal feeding. They are nutritional and health-care feed additives. The dominant flora and microecological balance in animals play an important role in reducing harmful intestinal products and housing odor. [0003] Generally speaking, the production process of probiotics can be divided into solid state fermentation and liquid state fermentation. Liquid fermentation refers to the fermentation process in which the fermentation medium is liquid. It has the advantages...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/225C12R1/125
Inventor 黄兴国杨承剑李宗军贺建华黄璜文利新
Owner CHANGSHA LVYE BIOTECHNOLOGY CO LTD
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