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Method of preparing nano slow release agent for simplifying animal FSH superovulation

A technology of superovulation and sustained release agent, which is applied in embryo engineering and the field of animal FSH superovulation control, to achieve the effect of strong repeatability, simplified superovulation procedure and stable chemical properties

Inactive Publication Date: 2008-02-27
YANGZHOU UNIV
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  • Abstract
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Problems solved by technology

The above phenomenon is especially serious when the ovulation number is increased by increasing the dose of PMSG

Method used

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Effect test

Embodiment Construction

[0017] In this implementation, mice are taken as an example, and the specific operation steps are as follows:

[0018] 1. Reagent preparation:

[0019] Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were purchased from Ningbo Hormone No. 2 Factory; self-made vesicle fluid; Duchenne's phosphate buffered solution (PBS); 0.9% normal saline

[0020] 2. Equipment:

[0021] 1ml syringe; cotton ball; fingertip

[0022] 3. Operation steps

[0023] 3.1 Selection of female mice

[0024] Select clean-grade Kunming white mice aged 6-7 weeks and weighing 25g-30g. It is raised in a suitable environment with an ambient temperature of 25 ± 5°C.

[0025] 3.2 Superovulation

[0026] 3.2.1 Synthesis of nano-scale sustained-release system:

[0027] Before superovulation in mice, prepare vesicle fluid:

[0028] Firstly, the fatty acid (palmitic acid, oleic acid or myristic acid) with a concentration of 0.5%-3% is dissolved and shaken at a pH value of 7-9, and after 8-12 ho...

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PUM

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Abstract

The invention discloses a making method of nanometer slow-release agent to simplify animal FSH superovulation in the embryo engineering technical domain, which comprises the following steps: 1) dissolving 0. 5%-3% fatty acid in the alkaline condition for 8-12h to make vesicle; 2) adjusting the grain size of vesicle and surface load; adding cationic surfactant in the vesicle liquid with weight rate of vesicle liquid and cationic surfactant at 1: 0. 05-0. 1; 3) adopting phosphoric acid buffer to adjust the grain size and surface load into 0. 05%-1. 5% vesicle slow-release agent. The invention simplifies the operation of superovulation without special request for instrument, which has strong repeatability.

Description

technical field [0001] The invention belongs to the technical field of embryo engineering, in particular to the technical field of controlling animal FSH superovulation. Background technique [0002] There are 200,000 to 400,000 oocytes on the ovary of mammals at birth, but 99% of the follicles are atresia during the entire life of the animal, and only 1% of the follicles mature and ovulate. In order to obtain more oocytes or embryos, at a certain stage of the estrous cycle of animals, through exogenous hormone treatment, increase the concentration of gonadotropins in the blood, reduce the atresia rate of developing follicles, and increase the number of early follicles developing to mature follicles, Stimulate oocytes that are temporarily in a dormant state to enter the mature development period, and then discharge them after maturation, so that many more oocytes can be obtained than under natural ovulation. This process is called superovulation (abbreviated as superovulatio...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K38/24A61P15/08
Inventor 丁家桐郭霞尹萍崔波季金强张舒谷亚轻俞凤燕
Owner YANGZHOU UNIV
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