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Method for preparing antihypertensive peptides by using rice separated protein

A blood pressure-lowering peptide and protein separation technology, which is applied in the fields of cardiovascular system diseases, fermentation, and drug combination, can solve the problems of high cost of separation methods and unsuitability for large-scale production, and achieve simple and easy source of raw materials, complete absorption, and process Scientific and reasonable effect

Inactive Publication Date: 2008-02-20
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the raw materials selected in these studies are animal proteins, and the separation methods are either too expensive, or the ash content in the peptide is high, which affects its activity, etc., which are not suitable for large-scale production.
The use of alkaline protease to hydrolyze rice protein isolates to produce hypotensive peptides has not been reported at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The alkaline protease (enzyme activity is 1,500,000 U / g) provided by Guangbo Nanning Pangbo Bioengineering Co., Ltd., the process conditions are: the concentration of rice protein isolate is 15%, the pH value is 8, the reaction temperature is 50 ° C, and the amount of enzyme added is 1 %, the reaction time is 120min. Determination of the inhibitory rate and concentration of rice antihypertensive peptide products to ACE, and calculation of IC 50 , and other indicators such as ash content are shown in Table 1.

[0019] Table 1 Main physical and chemical indicators of rice hypotensive peptide products

[0020] project

Embodiment 2

[0022] Alkaline protease (enzyme activity: 600,000 U / g) provided by Wuxi Genenco Bioengineering Co., Ltd. was used. The process conditions were: the concentration of rice protein isolate was 10%, the pH value was 9.5, the reaction temperature was 40°C, and the amount of enzyme added was 2 %, the reaction time is 180min. Determination of the inhibitory rate and concentration of rice antihypertensive peptide products to ACE, and calculation of IC 50 , and other indicators such as ash content are shown in Table 2.

[0023] Table 2 Main physical and chemical indicators of rice hypotensive peptide products

[0024] project

Embodiment 3

[0026] Alkaline protease (enzyme activity: 2.4AU / g) provided by Novozymes (China) Biotechnology Co., Ltd. was used. The process conditions were: the concentration of rice protein isolate was 10%, the pH value was 8.5, the reaction temperature was 45°C, and the amount of enzyme added 1%, the reaction time is 150min. Determination of the inhibitory rate and concentration of rice antihypertensive peptide products to ACE, and calculation of IC 50 , and other indicators such as ash content are shown in Table 3.

[0027] Table 3 Main physical and chemical indicators of rice hypotensive peptide products

[0028] project

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PUM

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Abstract

The invention discloses a method of preparing antihypertensive peptide with rice-separated protein. The method is technically characterized in that: rice-separated protein solution, the weight percentage viscosity (W / W) of which is 5-15 percent, is hydrolyzed by alkali proteinase. The process conditions of hydrolysis are: pH value: 8-10; reaction temperature: 40-50 DEG C; enzyme added: 1-2 percent; reaction time: 120-180 min; stirring enzymolysis. Then the hydrolysate is desalinated by macroporous adsorptive resin (NKA) and eluted by 40-80 percent of alcohol. The desalinated small peptide is concentrated or dried, thereby producing the small peptide that has angiotensin-converting enzyme (ACE) inhibitory activity. The invention is characterized in that: a new method of preparing antihypertensive peptide is provided; the technique is scientific, reasonable and easy; wide resources of the raw material are available.

Description

technical field [0001] The invention belongs to the field of food processing technology or pharmaceutical technology, and specifically relates to functional food processing technology, in particular to a method for preparing blood pressure-lowering peptide by using rice protein isolate. Background technique [0002] ACE inhibitors are one of the fastest-growing antihypertensive drugs, but the synthetic angiotensin-converting enzyme has adverse reactions, such as proteinuria and leukopenia, etc. In addition, it is easy to cause "drug withdrawal syndrome" after drug withdrawal , seriously threatening the life safety of patients. [0003] Antihypertensive peptides derived from food protein can completely overcome various side effects in drug treatment. It only has antihypertensive effect on hypertensive patients, but has no antihypertensive effect on normal blood pressure; these peptides are produced by enzymes under mild conditions Obtained by hydrolysis, it has high safety, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02A61P9/12
Inventor 陈季旺刘英杨国燕
Owner WUHAN POLYTECHNIC UNIVERSITY
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