Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier

A gold nanoparticle and arginine-rich technology, which is applied in the field of arginine-rich oligopeptide-gold nanoparticle cell transport carrier synthesis, can solve the problems of complex synthesis process, long reaction time, and difficulty in combining multiple molecules, etc. Simple operation, high transfer efficiency and mild conditions

Inactive Publication Date: 2010-05-19
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing methods of synthesizing gold nanocarriers using peptide chains as the delivery medium often require the use of polymers or proteins to stabilize gold nanoparticles, combined with delivery peptide chains, the synthesis process is often very complicated and requires a long reaction time , and it is difficult to combine multiple molecules to achieve multifunctionality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier
  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier
  • Method for synthesizing arginine-enriched polypeptide-gold nano particle cell transmission carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Provided is a method for synthesizing an arginine-rich oligopeptide-gold nanoparticle cell transport carrier. First, according to the Frens-Turkevich method, using sodium citrate to reduce chloroauric acid to prepare gold nanoparticles with a particle size of 30nm; the amino acid sequence is CALNN and CALNNGGRRRRRRR (CALNNR 8 ) of the oligopeptides are mixed, so that the molar concentration ratio of the two oligopeptides is 90%: 10%, to obtain the oligopeptide mixture; the oligopeptide mixture is added to a particle diameter of 30nm gold nano solution, so that the total molar concentration of the oligopeptide is 1mM, left to react at room temperature for 1h, adjusted the pH value of the solution to 3, so that the gold nanoparticles remained monodispersed in the solution, discarded the supernatant after centrifugation at 10000rpm, and then added a pH value equal to the supernatant 3 deionized water, and finally synthesized the arginine-rich oligopeptide-gold nanoparticle...

Embodiment 2

[0024] Make oligopeptide CALNN and CALNNGGRRRRRRR (CALNNR 8 ) molar concentration ratio is 0%:100%. Other steps are with embodiment 1

Embodiment 3

[0026] Allow the oligopeptide and the gold nanoparticle solution to react at room temperature for 1 hour, adjust the pH value of the solution to 6, keep the gold nanoparticle in the solution monodisperse, discard the supernatant after centrifugation at 10,000 rpm, and then add the supernatant, etc. amount of deionized water with a pH of 6. Other steps are the same

[0027] Example 1

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a synthesis method of an arginine-rich polypeptide-gold nanometer carrier. The invention uses a polypeptide surface decorative method and takes a polypeptide to stably cover gold nanometer grain and another type of polypeptide to realize cell cross-membrane transmission function of nanometer grain. The polypeptide-gold nanometer carrier with different transmission capacities can be obtained through regulating proportion of stable polypeptide and transmission polypeptide in primary mixture. The synthesis method has simple operation, short operation time and straightforward detecting method and the synthetic gold nanometer grain carrier has high efficiency of cell cross-membrane transmission.

Description

technical field [0001] The invention relates to a method for synthesizing an arginine-rich oligopeptide-gold nanoparticle cell transport carrier. technical background [0002] Due to its ultra-small size and specific properties (such as fluorescence, magnetism, and compactness), inorganic nanobiotransport systems provide new media and technical means for the acquisition of biochemical processes in living cells and medical targeted drug therapy (Nature Biotechnology, Nat. Biotechnol. 2001, 19, 1013-1017). Among them, the hybrid particles of biomolecules and gold nanoparticles can be used for real-time and dynamic observation of cells due to their biocompatibility and unique optical properties. Therefore, some studies have combined DNA, proteins, antibodies, polymer molecules and gold particles. Transmembrane transport (Science, Science, 2006, 312, 1027-1030; Biopolymer Chemistry, Bioconjugate Chem. 2004, 15, 482-490). In recent years, oligopeptide molecules have also been a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C07K17/14C07K7/08C07K7/06
Inventor 孙琳琳王振新
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com