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Composite culture medium for producing lincomycin, and production method for raising output and quality

A technology of lincomycin and culture medium, which is applied in the field of compound medium for producing lincomycin, and achieves the effects of less foam, stable quality of finished products, and coordinated, synchronous, stable and efficient production of hormones.

Inactive Publication Date: 2007-12-26
南阳普康药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems existing in the prior art, to provide a compound medium suitable for producing lincomycin that is more effective, reliable in use, low in cost and economical in use, and can improve production. And the production method of quality effectively solves the problem of improving the production efficiency of lincomycin, increasing yield and improving the quality of lincomycin, and reducing the B component of lincomycin. The molecular structural formula of said lincomycin is:

Method used

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  • Composite culture medium for producing lincomycin, and production method for raising output and quality
  • Composite culture medium for producing lincomycin, and production method for raising output and quality
  • Composite culture medium for producing lincomycin, and production method for raising output and quality

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preparation example Construction

[0052] The preparation of compound medium is carried out according to the following method steps:

[0053] First, take 2 / 3 of the amount of corn flour, corn starch, calcium lactate, and water to soak in the reaction tank according to the proportion of the formula, and add 2 / 3 of the α-amylase according to the proportion of the formula, and heat up Carry out the first stage of liquefaction at 59-61°C for 2 hours; then take 1 / 3 of the amount of corn flour, corn starch, calcium lactate, and water according to the proportion of the formula and add them to the reaction tank, and then add them according to the proportion of the formula Add 1 / 3 of α-amylase, raise the temperature to 84-86°C for the second stage of liquefaction for 1.5 hours; then lower the temperature of the liquefied liquid to 60°C, add glucoamylase and protease respectively according to the ratio of the formula, and keep warm for 7.5- 8.5 hours; when the reaction solution is viscous and fluid and dark yellow, lower...

Embodiment 1

[0056] The ratio of the composition of the present invention is: 70 kg of corn flour, 30 kg of corn starch, 0.02 kg of α-amylase, 1.0 kg of glucoamylase, 0.0002 kg of protease, 0.20 kg of calcium lactate and 260 kg of water. The prepared compound medium contains the following components: 32% glucose, 40% total sugar, 3.1% nitrogen, and 340 micrograms per milliliter of dissolved phosphorus.

Embodiment 2

[0058] The formula ratio of the present invention can also be: 75 kg of corn flour, 25 kg of corn starch, 0.02 kg of α-amylase, 1.1 kg of glucoamylase, 0.0004 kg of protease, 0.19 kg of calcium lactate and 280 kg of water. The prepared compound medium contains the following components (mass concentration): 33% of glucose, 42% of total sugar, 3.2% of nitrogen, and 360 micrograms per milliliter of dissolved phosphorus.

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Abstract

This invention relates to a composite culture medium for producing lincomycin, and a method for increasing productivity and quality of lincomycin. The composite culture medium is prepared from: corn powder, cornstarch, alpha-amylase, glucoamylase, protease, and methionine or sodium methionine or methioninamide. The method comprises: diluting the composite culture medium with water, adjusting the pH value, adding methionine or sodium methionine or methioninamide, and fermenting. The method can increase lincomycin productivity by 14%, and reduce the cost by 15%. The lincomycin product has a low lincomycin B content (below 1.0%), and low toxic or side effect, thus can be used as a starting material for producing lincomycin derivatives, lincomycin phosphate and lincomycin palmitate used in medical field and animal husbandry.

Description

1. Technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a compound medium for producing lincomycin and a production method for improving yield and quality. 2. Background technology [0002] Lincomycin (lincomycin) is an antibiotic discovered in the United States in 1962. It is obtained from the culture medium of Streptomyces varietal. coccus, viridans streptococcus and Streptococcus pneumoniae have good antibacterial effect, and have good antibacterial effect on anaerobic bacteria. Because of its strong penetrating power to tissues and cells, convenient application, no need for skin test, and remarkable anti-infection effect, it is widely used in medical treatment, animal husbandry and lincomycin derivative products clindamycin and clindamycin Starting material for phospholipid, clindamycin palmitate. Lincomycin contains two components, A and B, and its main component is component A, namely lincomycin, with the molecular formula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12P19/00
Inventor 李德文张小贝王冰段永平赵晴曾宪芳吴彦
Owner 南阳普康药业有限公司
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