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Biological tissue material for artificial esophagus and preparation method thereof

A biological tissue and artificial esophagus technology, which is applied in the field of human organs replacing artificial biological tissue materials and artificial esophagus biological tissue materials, can solve the problems of complex construction process, high technical requirements, and difficulty in realizing industrialization, and achieves the preparation process. Simple, low technical requirements, good elasticity

Inactive Publication Date: 2007-12-12
SICHUAN CANCER HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The recently developed tissue-engineered artificial esophagus has good performance in overcoming anastomotic leakage and stenosis, but its construction process is complicated and its technical requirements are high, so it is difficult to achieve industrialization

Method used

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  • Biological tissue material for artificial esophagus and preparation method thereof
  • Biological tissue material for artificial esophagus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The thoracic aorta of pigs killed in suicide farms was taken out, stored in iced saline, and quickly transferred to the laboratory. Remove adipose tissue and reticular connective tissue attached to the surface. Rinse repeatedly with a large amount of normal saline to remove blood and fat until no blood seeps out. Afterwards, sterilize and degrease with an aqueous solution of benzalkonium bromide with a mass concentration of about 0.03% for about 45 minutes, and then repeatedly rinse with phosphate buffer to remove benzalkonium bromide. The benzalkonium bromide aqueous solution is prepared by adding clear water to a commercially available benzalkonium bromide solution with a mass concentration of 5%.

[0026] The rinsed porcine aorta blood vessels and the cross-linking solution were placed in a shaker for cross-linking under the condition of gentle shaking, so that the cross-linking solution and the cross-linked tissue could contact more fully and evenly. The time betw...

Embodiment 2

[0030] The biological tissue material for the artificial esophagus prepared in this example is based on the porcine aorta and uses genipin as the cross-linking agent. The descending aortic blood vessel of the descending aorta, the mass concentration of benzalkonium bromide aqueous solution used for disinfection and degreasing is about 0.05%, the pH value of the cross-linking system is about 4.0, the pH value of the cross-linking liquid is about 4.0, and the pH value of the cross-linking liquid is about 4.0. The concentration of genipin is about 4.42 mmol of genipin in 100 ml of cross-linking solution; the cross-linking time is 8 days. Preparation of buffer: first prepare 0.1M citric acid solution, then prepare 0.1M sodium citrate solution, take 1 part of sodium citrate solution and add about 2.1 parts of citric acid solution to prepare citric acid / sodium citrate buffer solution. The amount of cross-linking solution is, per 1cm 2 The tissue was treated with 5ml-10ml cross-link...

Embodiment 3

[0033] The biological tissue material for the artificial esophagus prepared in this example is based on the porcine aorta and uses genipin as the cross-linking agent. The descending aortic blood vessel of the descending aorta, the mass concentration of benzalkonium bromide aqueous solution used for disinfection and degreasing is about 0.04%, the pH value of the cross-linking system is about 7.4, the pH value of the cross-linking liquid is about 7.4, and the pH value of the cross-linking liquid is about 7.4. The concentration of genipin is about 2.76 mmol of genipin in 100 ml of cross-linking liquid; the cross-linking time is 6 days. Buffer preparation: prepare 0.14M NaCl, 0.003M KCl, 0.01M NaCl in the same double distilled water 2 HPO 4 and 0.0016M of KH 2 PO 4 , and then adjust the pH to 7.4 with HCl. The amount of cross-linking solution is 5ml-10ml of cross-linking solution per 1cm2 of tissue.

[0034] The prepared cross-linked material is stored in an ethanol solution ...

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Abstract

The invention disclosed biologic tissue material is a biologic tissue material for artificial esophagus prepared from porcine aortic blood vessel as basal body and genipin as cross-linking agent. Its main operation for preparation is: cleaning aortic blood vessel took from alive killed pig, removing bloodstain and grease, and performing cross link treatment with crosslinked fluid formulated from buffer solution and genipin cross-linking agent, wherein genipin concentration in cross-linking system is 2.70-4.50mmol / 100ml, pH is 3.5-7.4, and temperature is 20-28deg.C.Biologic tissue material for artificial esophagus prepared by full cross link has good mechanical characteristics such as tenacity and elastic property like high molecular material, has natural structure suitable for peripheral cell, promote cellular adhesion growing on its surface, and is expected to reduce complication such as anastomotic leakage, stricture and necrosis in clinical application.

Description

technical field [0001] The invention relates to the technology of artificial biological tissue materials for human organs replacement, more specifically, relates to a technology of biological tissue materials for artificial esophagus, which belongs to the field of biological materials. technical background [0002] Some diseases of the esophagus, such as esophageal cancer, benign esophageal stricture and congenital esophageal atresia, are usually treated with surgery. The traditional method of surgical treatment is to replace the resected esophagus with other tissues and organs of the patient's own. This alternative treatment method is traumatic to the patient, and it will bring a series of complications such as anastomotic leak, stenosis and necrosis after the operation. At the same time, because part of the gastrointestinal tract is sacrificed to replace the esophagus, the patient often suffers from digestive disorders and nutrient absorption disorders. . For this reason...

Claims

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Application Information

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IPC IPC(8): A61L29/00
Inventor 任光国余喜讯万昌秀王志仪冯婷张小华
Owner SICHUAN CANCER HOSPITAL
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