Medicine composition for treating and preventing stomach and intestine tumor

A composition and tumor technology, applied in the field of medicine, can solve the problems of limited oncogene or tumor suppressor gene methylation or acetylation research, etc., and achieve the effects of low price, reducing toxic and side effects, and improving anti-tumor effect.

Inactive Publication Date: 2007-12-05
房静远 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of research on epigenetic modification, especially on human gastrointestinal tumors, there are many reports, but they are mainly limited to the methylation or acetylation of a single oncogene or tumor suppressor gene

Method used

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  • Medicine composition for treating and preventing stomach and intestine tumor
  • Medicine composition for treating and preventing stomach and intestine tumor
  • Medicine composition for treating and preventing stomach and intestine tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Colorectal cancer cell lines SW1116 and Colo-320 (purchased from Shanghai Institute of Cellular Science, Chinese Academy of Sciences) were routinely cultured in RPMI 1640 medium, and quantitative RT-PCR, sequencing after bisulfite modification or methylation-specific PCR (MSP) And Western blotting, chromatin immunoprecipitation (ChIP) to analyze the changes of related gene expression, DNA methylation and histone acetylation before and after intervention, and flow cytometry (FCM) to analyze the changes of cell cycle . NaBu (5mmol / L), 5-aza-dC (2μmol / L), and the pharmaceutical composition of the present invention (in which NaBu 5mmo / L, 5-aza-dC 2μmol / L) were intervened for 24 hours respectively. The control group was without any treatment. After synchronization treatment, the cell cycle was detected by flow cytometry, and the tumor suppressor gene p16 was detected by RT-PCR INK4A and p21 WAF1 mRNA expression, p16 analysis by co-immunoprecipitation INK4A and p21 WAF1 ...

Embodiment 2

[0034] The chemical carcinogen dimethylhydrazine (1,2-dimethylhydrazine, DMH) was used to induce colorectal cancer in ICR mice. The mice were female inbred S-ICR mice weighing 18-20 g (purchased from the Chinese Academy of Sciences in Shanghai). During the modeling period, NaBu, 5-aza-dC, 5-aza-dC and NaBu pharmaceutical compositions were used to intervene in the tumorigenesis process, and the pathological changes of the mouse intestine were analyzed.

[0035] Grouping of mice and drug intervention

[0036] 1) blank control group,

[0037] 2) NaBu control group,

[0038] 3) 5-aza-dC control group,

[0039] 4) drug combination control group,

[0040] 5) DMH control group,

[0041] 6) DMH+NaBu,

[0042] 7) DMH+5-aza-dC,

[0043] 8) DMH + the drug of the present invention (NaBu, 5-aza-dC).

[0044] About dosage and route of administration:

[0045] The carcinogen DMH is a white powder crystal, and it is made into a 0.4% solution with sterile normal saline before each inje...

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Abstract

The present invention belongs to the field of medicine technology, and is especially one kind of medicine composition containing 5-nitrogen deoxycytidine and short chain fatty acid butyrate and its application in preventing and treating gastrointestinal tumor. Experiments show that the medicine composition of the present invention can affect the methylation and acetylation of apparent genetic modification DNA, and further affect the gene expression of cell line of gastric cancer and/or large intestinal cancer or even change the cell period and inhibit tumor growth. The present invention has obvious tumor preventing and treating effect, no obvious toxic side effect and low cost.

Description

field of invention [0001] The invention belongs to the technical field of medicine and relates to a pharmaceutical composition for preventing and treating gastrointestinal tumors, in particular to a pharmaceutical composition containing 5-azodeoxycytidine and short-chain fatty acids and its application in preventing and treating gastrointestinal tumors. Background technique [0002] Gastric cancer and colorectal cancer are common malignant tumors in my country. The incidence rate remains high, and most of them are in the middle and late stage when symptoms appear, and the clinical treatment effect is often unsatisfactory. Therefore, how to prevent the occurrence of gastric cancer and colorectal cancer has become a top priority. Studies have shown that epigenetic modifications can affect gene transcriptional activity without involving changes in DNA sequence. Abnormalities of epigenetic modification often occur in the process of gastrointestinal tumorigenesis, such as the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7068A61K31/19A61K9/00A61P35/00
Inventor 房静远陆嵘王霞
Owner 房静远
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