Modified coffee, method of roasting coffee bean, coffee-like supplement and auxiliary food
A roasting method and coffee bean technology are applied in the field of improving coffee, and can solve the problems of poor flavor, delicious components, and loss of flavor components in decaffeinated coffee products.
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[0289] HPLC analysis of niacin contained in roasted coffee beans
[0290] At a roasting temperature of 200°C, the Maillard reaction product, which is the basis of the aroma of coffee products, was produced, and then, the most suitable roasting time for the thermal conversion of trigonelline in green beans to niacin was determined to be 40 minutes using Equation 1 . Under these conditions, 200 g of green coffee beans (produced in Brazil) were roasted and ground for 20 seconds with a coffee grinder. The obtained 10g powder was soaked and decocted with 30ml hot water. After centrifugation at 3000 rpm for 5 minutes, 1 ml of the supernatant was injected into Sep-Pak PlusCl8 (trade name, manufactured by Wootas Co., Ltd.), and eluted with 3 ml of acetonitrile / pure water (7 / 93). 5 μl of the eluate were analyzed by HPLC. The experimental conditions are as follows. A liquid delivery pump (trade name BIP-1 / manufactured by JASCO Corporation), a multi-wavelength ultraviolet absorption...
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