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Selenium-rich saccharomyces cerevisiae, selenium-rich yeast product and their production process

A technology of selenium-enriched Saccharomyces cerevisiae and selenium-enriched yeast is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc. The effect of high selenium content, stable selenium resistance and fast growth rate

Active Publication Date: 2007-10-03
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been many studies on selenium-enriched yeast at home and abroad, but few of them can be mass-produced. The main problems are: (1) the selenium content is low, generally below 1000mg / kg, and the high one is 1500mg / kg; (2) inorganic The selenium content is low, and some are just simple yeast and selenium adsorption, and the selenium content of methionine is lower; (3) some methods are not suitable for large-scale production, and the repeatability is very poor, and some can be produced, but the yield is very low; (4) for Selenium utilization is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the screening of saccharomyces cerevisiae strain of the present invention

[0046] 1) A variety of yeast strains with different sources and uses are inoculated into different concentrations of selenium-containing medium, and the strains that can grow in high-selenium medium are screened. The strains resistant to selenium were selected as the strains for mutagenesis.

[0047] 2) Inoculate the selenium-resistant strains on the medium containing different doses of nitrosoguanidine for 24 hours of mutagenesis.

[0048] 3) Separating and screening the mutagenized yeast strains, using shake flasks and small fermenters for fermentation to select the ones with high selenium content, fast growth rate, high yield and high utilization rate of selenium on the medium containing selenium The high-selenium-resistant selenium-enriched yeast strain is the high-selenium-resistant selenium-enriched yeast strain S205.

[0049] 4) Screening medium formula

[0050] The wort ...

Embodiment 2

[0053] Embodiment 2: the production of selenium-enriched yeast product-Scheme 1

[0054] The production process of selenium-enriched yeast with high selenium content: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter seed cultivation → fermenter cultivation → separation → drying → packaging. The specific process is as follows:

[0055] 1) Incline strain culture: Inoculate the selenium-rich yeast strain with high selenium resistance to a sugar content of 5 to 25% by weight, a selenium (selenate or selenite) concentration of 10 μg / ml, and a pH value of 4 ~6.5 wort solid slant, incubate 24~48 hours at 26~35℃.

[0056] 2) First-level liquid seed culture: Inoculate 1 to 4 rings of yeast strains on the inclined plane to 100 to 500 ml of 5 to 25% by weight of sugar content and 10 μg of selenium (selenate or selenite) concentration. / ml, pH value of 4 to 6.5 wort special culture bottle, cultured at 26 to 35 ℃ for 24 to 48 hours.

[0057...

Embodiment 3

[0063] Embodiment 3: the production of selenium-enriched yeast product-Scheme 2

[0064] The production process of selenium-enriched yeast with high selenium content: slant culture → primary liquid seed cultivation → secondary liquid seed cultivation → fermenter seed cultivation → fermenter cultivation → separation → drying → packaging. The specific process is as follows:

[0065] 1) Slant strain cultivation: inoculate the selenium-rich yeast strains resistant to high selenium to a sugar content of 5 to 25% by weight, a selenium (selenate or selenite) concentration of 300 μg / ml, and a pH value of 4 ~6.5 wort solid slant, incubate 24~48 hours at 26~35℃.

[0066] 2) First-level liquid seed culture: inoculate 1 to 4 rings of yeast strains on the inclined plane to 100 to 500 ml of 5 to 25% by weight of sugar content and 300 μg of selenium (selenate or selenite) concentration. / ml, pH value of 4 to 6.5 wort special culture bottle, cultured at 26 to 35 ℃ for 24 to 48 hours.

[00...

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PUM

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Abstract

The present invention relates to one kind of selenium-rich Saccharomyces cerevisiae, selenium-rich Saccharomyces cerevisiae product and their production process. Of the selenium-rich Saccharomyces cerevisiae product, each kilogram contains selenium in 4000-8000 mg, and organoselenium accounts for 90-99 wt% of the total selenium content. The process of producing selenium-rich Saccharomyces cerevisiae product includes the following steps: 1. fermenting the selenium-rich Saccharomyces cerevisiae of the present invention in culture medium containing selenium in 10-300 mcg / ml at 26-35 deg.c; and 2. drying the fermented Saccharomyces cerevisiae liquid to obtain the selenium-rich Saccharomyces cerevisiae product. The selenium-rich Saccharomyces cerevisiae product is safe and non-toxic, and may be used in medicine, food, feed and other fields.

Description

technical field [0001] The invention relates to a selenium-enriched Saccharomyces cerevisiae, a selenium-enriched yeast product obtained from the selenium-enriched Saccharomyces cerevisiae and a production method thereof. Background technique [0002] Selenium is an essential trace element for humans and animals. It is an important part of various enzymes. Selenium is an important component of glutathione peroxidase. Through oxidation and reduction, it protects biofilms from damage and maintains normal cells. Features. Selenium can also enhance immunity, protect cardiovascular, promote growth, anti-tumor, detoxify, regulate metabolism and other functions. The endemic diseases caused by selenium deficiency in my country include Keshan disease and Kaschin-Beck disease. Selenium-enriched yeast has an obvious effect on supplementing some diseases caused by selenium deficiency. In addition, yeast is rich in nutrients and can play a synergistic effect. [0003] In recent years,...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/865
Inventor 俞学锋李知洪余明华姚娟张彦
Owner ANGEL NUTRITECH CO LTD
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