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Method of detecting KLK1 gene rs5517 polymorphism correlated to primary hypertension

A technology of essential hypertension and genes, applied in the KLK1 gene rs5517 polymorphism kit, to prepare reagents for diagnosing essential hypertension, and in the field of reagents for judging the relative risk of suffering from essential hypertension, can solve the problem There are no problems such as patent reports, and the effect of preventing the occurrence of high blood pressure, high prevalence, and deepening understanding

Inactive Publication Date: 2007-09-12
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI +1
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Problems solved by technology

At present, there are no patent reports on the relationship between the rs5517 mutation of the KLK1 gene and essential hypertension, as well as the detection method, kit, and application prospects of the mutation in forecasting and new drug development.

Method used

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  • Method of detecting KLK1 gene rs5517 polymorphism correlated to primary hypertension
  • Method of detecting KLK1 gene rs5517 polymorphism correlated to primary hypertension
  • Method of detecting KLK1 gene rs5517 polymorphism correlated to primary hypertension

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Embodiment Construction

[0040] 1. Test samples and methods for detecting rs5517 polymorphism

[0041] The rs5517 polymorphism was detected by PCR combined with the restriction fragment length polymorphism method.

[0042] 1.1 Acquisition of test samples

[0043] All DNA samples and clinical data are from the International Collaborative Study of Cardiovascular Disease in Asia (InterASIA). The program was approved by the local ethics committee, and each participant voluntarily signed an informed consent form. A total of 15,838 representative volunteers between the ages of 35 and 74 participated in this plan. We selected 2,411 independent volunteers from 9 provinces and cities including Jilin, Beijing, Shandong, Shaanxi, Guizhou, Fujian, Hubei, Sichuan, and Guangxi. Hypertensive individuals and 2348 independent control individuals matched by sex, age, and place of residence. Participants filled out questionnaires in detail, including personal disease history, family history, smoking and drinking hist...

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Abstract

The invention relates to a method for detecting KLK1gene rs5517 polymorphism relating to essential hypertension. The method includes detecting A / G polymorphism located at 3812 bp of KLK1 gene coding sequence, in which, the distributed frequency of allele A has positive correlation with the risk rate of essential hypertension. The invention also involves the kits to detect rs5517 polymorphism of KLK1 gene.

Description

technical field [0001] The invention relates to a gene related to essential hypertension and its detection method and application. More specifically, the present invention relates to the detection method and application of KLK1 gene rs5517 polymorphism related to essential hypertension, for example, in preparing reagents for diagnosing essential hypertension or judging the relative risk of suffering from essential hypertension use in reagents. The invention also relates to a kit for detecting the rs5517 polymorphism of KLK1 gene related to essential hypertension. Background technique [0002] The English name of tissue kallikrein gene is kallikrein 1, renal / pancreas / salivary (abbreviation: KLK1), and its protein product is tissue kallikrein. The KLK1 gene was cloned and located on human chromosome 19q13.3 in 1988. It is about 5kb long and includes 5 exons. [0003] Kallikreins are a group of serine proteases present in different tissues and body fluids, including plasma k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 顾东风赵维艳王来元李宏帆王亚平陈恕凤
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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