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Antisense oligonucleotide-carbon nanotube medicament vector

A technology of oligonucleotides and carbon nanotubes, applied in the field of drug carriers and their preparation, can solve the problems of low transfection rate and ineffective effect of tumor cells, and achieve high transfection rate, obvious effect and simple preparation method Effect

Inactive Publication Date: 2007-08-15
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

[0011] The technical problem to be solved by the present invention is to provide an antisense oligonucleotide-carbon nanotube drug carrier, which can effectively improve the loading and targeting of antisense oligonucleotides, so as to overcome the problem of using antisense oligonucleotides alone. Nucleotide drug transfection rate is low, the effect of inhibiting tumor cells is not obvious, etc.

Method used

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Embodiment 1

[0045] 1. Preparation of acidified MWNTs

[0046] MWNTs were placed in mixed acid (H 2 SO 4 with HNO 3 The volume ratio is 3:1), sonicated for 16 hours. The resulting MWNTs suspension was filtered with a 100 nm microporous membrane, and rinsed with twice distilled water until neutral. It was redispersed in double distilled water, and the large-particle MWNTs were removed by centrifugal filtration (7000rpm, 5min) several times to obtain MWNTs with length distribution between 100-300nm and -COOH modified on the tube wall (infrared spectrum Figure 2 , TEM Figure 3).

[0047] 2. Preparation of PEI-MWNTs aqueous phase system

[0048] Preparation principle: use the electrostatic force between groups.

[0049] 0.5 mg MWNTs were mixed with 5 ml 1% polyethyleneimine (PEI). After ultrasonication for 15 min, the resulting mixture was filtered with a 200 nm microporous membrane to remove excess unreacted PEI, and then dispersed into PBS with pH=7.2 to obtain a stable PEI-MWNTs aque...

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Abstract

This invention relates to an antisense oligonucleotide-carbon nanotubes medicine carrier which comprises carbon nanotube casing and antisense oligonucleotide. The preparation method comprises oxidizing multi-wall carbon nanotube with strong acid to carry carboxyl on the shell of pipe, cutting to 50-200nm with diameter of 10-50nm with ultrasound, adding poly-cationization bichloride to embellish on shell of carbon nanotube, and assembling antisense oligonucleotide to carbon nanotube. The preparation method is simple and rapid. The medicine carrier has high transfection efficiency, high target effect, and good effect in inhibiting tumor cell. Targeted bimolecular or medicine with carbon nanotube as carrier material aim at different biological system can be applied for treating tumor or genetic diseases.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a drug carrier composed of oligonucleotide and nanometer material and its preparation method and application. Background technique [0002] Selecting a suitable target gene as an attack point and a suitable carrier to increase the expression of drugs in vivo are two major issues encountered in the study of tumor therapy. However, the progress made in the molecular biological characteristics of tumors and biocompatible chemical materials in the past 20 years has laid a good foundation for the selection of target genes. [0003] Human telomeres are repetitive DNA sequences (TTAGGG) located at the ends of chromosomes, which play an important role in maintaining chromosomal stability. The change and stability of telomeres are closely related to cell aging and canceration. Telomerase is a special ribonucleoprotein polymerase, which can use its own RNA as a template to sy...

Claims

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Application Information

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IPC IPC(8): A61K47/26A61K47/04A61K48/00A61P35/00
Inventor 贾能勤连琼
Owner SHANGHAI NORMAL UNIVERSITY
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