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Method and device for plants carbon isotope labelling

An isotope labeling, plant carbon technology, applied in the field of isotope tracing, can solve the problems of difficult carbon dioxide gas leakage, incompatibility, complexity, etc., and achieve the effects of simple operation method, improved utilization efficiency, and simple device structure

Inactive Publication Date: 2007-08-15
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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Problems solved by technology

[0003] In the 1960s, British soil scientist Jenkinson designed a device for producing carbon-14 isotope-labeled plants, but it was complicated and inconvenient to operate, and the pressure control method in the box had defects, especially when adding When hydrochloric acid and carbon-14 labeled sodium bicarbonate, it is difficult to ensure the prevention of leakage due to the violent reaction
In addition, because the device uses a liquid culture method for plant production, it is not only easy to breed non-target organisms such as algae, but also may be different from plants grown in the soil, and some woody plants are even difficult to grow normally in a liquid culture solution; and the method Also not suitable for studying the amount of organic matter carbon exuded by plant roots
Many researchers in China usually use the intermittent labeling method, that is, plants in soil pots are grown under natural air environment conditions, and the plants are moved into the labeling box at regular intervals for carbon isotope labeling for one day. This method also has difficulties in strictly controlling labeling. The leakage of carbon dioxide gas is also not suitable for studying the amount of organic carbon secreted by plant roots, and the labeling intensity of carbon isotopes in the labeled plant materials is not uniform, so it is not suitable for studying the organic carbon of labeled plants in soil. decomposition and transformation

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  • Method and device for plants carbon isotope labelling

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Embodiment Construction

[0023] The method for plant carbon isotope labeling of the present invention comprises the following steps:

[0024] A. Add soil to the cultivation tray, take fresh surface soil (0-20 cm) equivalent to 40-60 kg of dry weight and low organic matter content, remove visible plant residues, and pass through a 2 mm aperture sieve after proper air drying , spray the mixed solution of ammonium sulfate, sodium dihydrogen phosphate and potassium chloride evenly in the soil, and the amount of nitrogen, phosphorus and potassium applied are 80, 20 and 80 mg / kg soil respectively, and the soil is divided into long × In a cultivation tray with a width of 45 x 15 cm and a height of 45 x 15 cm, 13-15 kg of soil is placed in each pan. Wash the seeds of rice or corn with water first, place them in a petri dish lined with filter paper, add 10 ml of distilled water, and place the petri dish at 25°C for 3-4 days to germinate the seeds. Sow germinated seeds such as rice or corn into the cultivation...

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Abstract

The invention discloses a method and device for marking plant carbon isotopes. The method comprises following steps: sowing seed in the cultivating disk which located in the soil; putting cultivating disk into air- tight box; adding sodium carbonate marked with carbon-14 or carbon- 13 under air- tight condition, carrying out reaction with hydrochloric acid in reaction tank to generate carbon dioxide for plant photosynthesis. The carbon dioxide in box is mixed with electrical fan, and light is replenished with daylight lamp, the temperature and humidity are reduced by using cooling tube, and water is added into cultivating disk with fluid infusion bottle. The plant marked with carbon- 13 or carbon- 14 is got after cultivation. The invention is characterized by simple and safe operation, prevention of carbon marked with carbon isotopes escape, increased utilization coefficient of carbon isotopes, suitability for carbon isotopes mark for various plants, especially for woody plant.

Description

technical field [0001] The invention relates to the technical field of isotope tracer, more specifically to a plant carbon isotope labeling method, and also relates to a plant carbon isotope labeling device. The invention is suitable for researchers in institutions of higher learning and scientific research units to carry out carbon-14 or carbon-13 isotope labeling and tracing research on plants. Background technique [0002] Due to its high sensitivity, isotope tracer technology is called another great invention after the invention of microscope technology, and has been widely used in various fields of biology research. In soil science research, soil organic matter is not only an important material basis for soil fertility, but also plays an important role in maintaining soil aggregate structure and water retention and ventilation performance. Research on the cycle and accumulation of soil organic carbon is important for maintaining soil fertility and sustainable agricultur...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00A01G7/02A01G9/18A01G9/00G01N1/00G09B23/28G01V15/00
Inventor 肖和艾吴金水李玲唐国勇
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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