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Methods and compositions for preventing biofilm formation, reducing existing biofilms, and for reducing populations of bacteria

A composition and biofilm technology, applied in botany equipment and methods, detergent compositions, chemical instruments and methods, etc., can solve the problems of company reputation damage and food processing enterprises spending a lot of money

Inactive Publication Date: 2007-07-18
BIOFILMS STRATEGIES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These new directives, along with the high number of recalls, cost food processors a lot of money, caused considerable pain, and damaged the company's reputation

Method used

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  • Methods and compositions for preventing biofilm formation, reducing existing biofilms, and for reducing populations of bacteria
  • Methods and compositions for preventing biofilm formation, reducing existing biofilms, and for reducing populations of bacteria
  • Methods and compositions for preventing biofilm formation, reducing existing biofilms, and for reducing populations of bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1: Cell-free fermented product

[0097] As shown in Figure 5, cell-free fermentations of four bacteria were prepared. The bacterial species used to produce the ferment were Lactococcus lactis subsp. lactis (ATCC #11955), Pediococcus lactis (ATCC #25742), Lactobacillus acidophilus (ATCC #4356) and Lactobacillus sake (ATCC #15521). A culture of each bacterium was placed on skim milk powder and incubated at 37°C for 18h. After culturing, the whey fraction is separated from the curd fraction. The whey was then centrifuged at 25,000 rpm for 10 minutes. It was then filtered using a Millipore (Billerica, MA) sterile filter with a pore size of 0.2 μm. In this way a sterile cell-free ferment is obtained.

Embodiment 2

[0098] Embodiment 2: Control

[0099] This embodiment is shown as an example in FIG. 6 . Five stainless steel sheets (1in 2 ;6.5cm 2 ) into a sterile petri dish, and sterile brain heart infusion (brain heart infusion, BHI) medium and LM were added to the petri dish. Incubate at 35°C for 6h to allow LM to attach to the surface of the steel sheet. Remove the stainless steel piece from the Petri dish using sterile forceps and rinse carefully with 1% sterile peptone medium. Then put the steel sheet on a Petri dish containing 1% sterile peptone medium and seal it with Parafilm membrane, and incubate at 35°C for 16h to grow LM biofilm. The steel piece was then shaken in a sterile urine cup with sterile glass spheres and 10 mL of Butterfield's phosphate buffered saline to remove biofilm on the steel piece. Samples were diluted appropriately, plated using complete plate count agar and incubated at 35°C for 24h and counted.

Embodiment 3

[0100] Embodiment 3: Encapsulation research

[0101] This embodiment is exemplarily shown in FIG. 7 . Five stainless steel sheets (1in 2 ;6.5cm 2 ) into a sterile cell-free fermentation of Pediococcus lactis, Lactococcus lactis subsp. lactis, Lactobacillus acidophilus and Lactobacillus sake and kept at room temperature (about 20° C.) for 1 h. The steel piece was then placed in a sterile petri dish and sterile brain heart infusion medium and LM were added to the dish. Incubate at 35°C for 6 hours to allow LM to attach to the surface of the steel sheet. Remove the stainless steel piece from the Petri dish using sterile forceps and rinse carefully with 1% sterile peptone medium. Then put the steel sheet on a petri dish containing 1% sterile peptone medium and seal it with Parafilm membrane, and incubate at 35°C for 16h to grow LM biofilm. The steel piece was then shaken in a sterile urine cup with sterile glass spheres and 10 mL of Butterfield's phosphate buffered saline to ...

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Abstract

Disclosed herein are compositions and methods for preventing biofilm formation, reducing existing biofilm, and / or reducing populations of pathogenic, indicator, and spoilage bacteria. In one example, disclosed are cell-free fermentates using Lactobacillus species, Pediococcus species, and / or Lactococcusspecies used separately, in combination, or combined with extract from Delisea pulchra marine algae.

Description

technical field [0001] Disclosed herein are methods and compositions for preventing biofilm formation, reducing existing biofilms, and / or reducing bacterial populations. Background technique [0002] To better quantify the health impact of foodborne illnesses, the US Centers for Disease Control (CDC) conducted an assessment. Mead et al collected and analyzed information from various surveillance systems and other sources (Food-Related Illness and Deathin the United States, Centers for Disease Control and Prevention, Atlanta, Georgia, USA, 2003). The report estimates that foodborne illnesses sicken approximately 76 million people, hospitalize 325,000, and kill 5,000 in the United States each year. Known pathogens have sickened approximately 14 million people, hospitalized 60,000, and killed 1,800. Three pathogens, Salmonella, Listeria and Toxoplasma, are responsible for 1,500 deaths per year, more than 75% of deaths caused by known pathogens, while unknown factors are Caus...

Claims

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Application Information

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IPC IPC(8): A61K35/66A61K35/74A61K35/20C12P1/00C12P1/04A01N63/20A61K35/744A61K35/747A61K45/00A61K45/06C11D3/48C11D7/40
CPCA61K35/747A23B7/154A01N63/02A23L3/3463A61K36/064A61K35/744A23B7/155A23B7/16C11D3/48A61K45/06A23B4/10A23B4/20A23B4/22C11D7/40A01N63/20
Inventor S·M·拉塞尔S·伯韦尔
Owner BIOFILMS STRATEGIES INC
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