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Novel acid-producing Klebsiella bacterium LF-1 and uses thereof

A Klebsiella, LF-1 technology, applied in the direction of bacteria, medical raw materials derived from bacteria, microorganisms, etc., can solve the problem of TMPD toxicity reduction and achieve the effect of solving poisoning

Inactive Publication Date: 2009-10-14
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no effective way to reduce the toxicity of TMPD in Oxytropis glacialis

Method used

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  • Novel acid-producing Klebsiella bacterium LF-1 and uses thereof
  • Novel acid-producing Klebsiella bacterium LF-1 and uses thereof
  • Novel acid-producing Klebsiella bacterium LF-1 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation and screening of Klebsiella oxytocia LF-1

[0037] 1. Soil sample

[0038] Collected from the environmental soil where locoweeds grow in Tibet, Inner Mongolia, Qinghai and other places;

[0039] 2. Isolation and screening of bacterial strains

[0040] Take 20g~30g of soil from four sources in the growth environment of locoweed to inoculate the seeds, incubate at 37°C for 24 hours, pick out a single colony and inoculate it in ordinary broth containing 50mg / L TMPD, incubate at 37°C for 48h, and preserve the strains Inoculate the strains preserved in the previous step in the common broth containing 100mg / L TMPD, and cultivate them at 37°C for 48h; repeat the above process, each culture will increase the content of TMPD, reduce the content of organic nutrients in the culture solution, Save the strain. After 5 times of cultivation, when the content of TMPD in the medium increased to 300mg / L, the strains were stored at 4°C for later use; the above-ment...

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PUM

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Abstract

The invention discloses Klebsiella oxytoca LF-1, which was preserved in the China Center for Type Culture Collection on November 20, 2006, and its preservation numbers are respectively CCTCC NO: M 206126; the Klebsiella oxytoca Bacteria LF-1 can effectively degrade phytotoxins, especially the toxicity of the toxic alkaloid 2,2,6,6-tetramethylpiperidone (TMPD) extracted and separated from the above-ground parts of Oxytropis glaciens, which is of great importance to the thorough Solve the poisonous damage of Oxytropis glacier, and play a positive role in the biological control of grassland poisonous weeds and the research of grassland ecology.

Description

technical field [0001] The invention relates to a new bacterial strain of Klebsiella oxytoca and the application of the bacterial strain in the preparation of phytodegradant toxin medicine. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Astragalus and Oxytropis in the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 . In recent years, important progress has been made in domestic and foreign research on the hazards of locoweeds, toxic components, toxicological mechanisms, prevention and utilization, but so far there is no effective method to control the spread of locoweeds, which has caused the problem of locoweed poisoning to become more and more serious. It has become a serious problem for gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/74C12R1/22
Inventor 王建华李勤凡王岚峰耿果霞
Owner NORTHWEST A & F UNIV
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