Rhizopus arrhizus epiphyte and application of the same in puer tea production
A technology of Rhizopus oligoides and fungi, which is applied to Rhizopus oligoides fungi and its application in Pu-erh tea production, and achieves the effects of shortening processing time, improving quality, and stabilizing and improving quality.
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Embodiment 1
[0012] Embodiment 1: the purification of Prhi501 bacterial classification
[0013] From the collected Pu'er tea samples, 48 strains of Rhizopus aligiosus strains were obtained by known plate separation (culture medium using Rhizopus isolation and purification medium). The rhizopus purification medium is: 200g of potatoes, 20g of glucose, 20g of agar, 1000ml of distilled water, and natural pH. Peel the potatoes, cut them into pieces, cook them for half an hour, then filter them with gauze, add agar and sugar, melt them and add water to 1000ml. Sterilize at 121°C for 20 minutes.
[0014] The purification method is as follows:
[0015] (1) Melt the solid medium (potato dextrose agar medium), cool it to 45°C, pour it into a sterile petri dish, about 15-20 ml per dish, shake it slightly, let it stand, and make it into a plate for later use.
[0016] (2) Take 2g of the tea sample to be separated, add it to a mortar with 98ml of sterile water beside the flame and grind it, and w...
Embodiment 2
[0021] Embodiment 2: the production application of Rhizopus oligorrhizae fungus
[0022] The maocha is moist-heated according to the traditional method, and then inoculate the maocha with 0.1-0.8% of Prhi501 by weight to let it ferment. Increased secretion of a large amount of glucoamylase. These enzymes oxidize and hydrolyze the ingredients contained in tea leaves. Among them, reducing sugars, soluble carbohydrates and hydrated pectin showed an increasing trend. However, at this time, in addition to Prhi501, a large number of other microorganisms began to grow in the tea, and the polysaccharide hydrolyzate was used by these many microorganisms for growth and reproduction. Therefore, the content of soluble carbohydrates is less than that of large-scale Pu-erh tea production and simulated large-scale production. At the same time, the content of crude fiber in the bulk tea samples is also less than that of the traditional treatment, which shows that the process of inoculating ...
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