Porous integrated substrate of carrying protein solid and its preparation
A protein and overall technology, applied in the direction of carrier binding/immobilization of peptides, peptides, specific peptides, etc., can solve the problems of long time consumption and low group activity, and achieve shortened immobilization time, high reactivity, and improved mass transfer and the effect of convection
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Embodiment 1
[0034] 1. Preparation of monolithic matrix: press figure 1 As shown, acryloylsuccinimide (NAS) and acrylamide were selected as monomers, and ethylidene dimethacrylic acid (EDMA) was used as a cross-linking agent to synthesize a porous monolithic matrix. The inner wall of the capillary is pre-modified by a silane reagent (3-trimethoxysilyloxypropyl methacrylate, γ-MAPS) with a double bond functional group, and monomers are polymerized in it and fixed on the capillary wall by chemical bonds. Succinimide on NAS is used as an active reactive group to derivatize and immobilize protein molecules. Specifically, such a porous material is polymerized in-situ in a capillary to form a monolithic column and applied to substrate hydrolysis experiments. Weigh 62.5mg of NAS, 62.5mg of acrylamide and 125mg of EDMA, dissolve in 750mg of cyclohexanol / dodecanol (mass ratio 85 / 15) mixed solvent, heat to 60°C, oscillate and mix, cool to 25°C , add 2.5mg of initiator AIBN, after passing nitrogen f...
Embodiment 2
[0041] 1. Preparation of monolithic matrix: press figure 1 As shown, acryloylsuccinimide (NAS) and acrylamide were selected as monomers, and ethylidene dimethacrylic acid (EDMA) was used as a cross-linking agent to synthesize a porous monolithic matrix. The inner wall of the capillary is pre-modified by a silane reagent (3-trimethoxysilyloxypropyl methacrylate, γ-MAPS) with a double bond functional group, and monomers are polymerized in it and fixed on the capillary wall by chemical bonds. Succinimide on NAS is used as an active reactive group to derivatize and immobilize protein molecules. Specifically, such a porous material is polymerized in-situ in a capillary to form a monolithic column and applied to substrate hydrolysis experiments. Weigh and weigh the monomer and porogen in the ratio shown in Table 2, heat to 80°C, oscillate and mix, cool to 25°C, add initiator AIBN, blow nitrogen for 1min, and ultrasonically degas for 5min. At room temperature, inject the above solut...
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