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Cluster hair wheat-serine/threonine kinase gene and its coded protein

A technology of threonine kinase and gene encoding, which is applied in the field of serine/threonine kinase gene, can solve the problems of rapid change of pathogenic bacteria race and loss of powdery mildew resistance, etc., and achieve the effect of variety improvement and powdery mildew resistance

Inactive Publication Date: 2008-10-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most economical and effective way to control powdery mildew is to use disease-resistant varieties. However, due to the rapid change of pathogenic species, most of the existing varieties in my country have gradually lost their resistance to powdery mildew.

Method used

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  • Cluster hair wheat-serine/threonine kinase gene and its coded protein
  • Cluster hair wheat-serine/threonine kinase gene and its coded protein
  • Cluster hair wheat-serine/threonine kinase gene and its coded protein

Examples

Experimental program
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Embodiment Construction

[0025] 1. Using barley gene chips to screen for disease-resistant gene analogs of T. villosa

[0026] The seeds of disease-resistant A. villosa (known public materials, Chen P D, Qi L L, Zhou B, et al. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS / 6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet, 1995, 91 : 1125-1128.) Sowed in a petri dish to germinate, transplanted to pots after dew white, and shake off the mixed powdery mildew spores (taken from the mixed spores of wheat powdery mildew in the field under natural conditions in Nanjing) on ​​the seedlings at the three-leaf stage Induce on. The disease-resistant T. villosa was sampled 24, 48, and 72 hours after powdery mildew inoculation, and RNA was extracted separately (extracted with Trizol reagent from Invitrogen Company) and mixed in equal amounts to form an experimental group; non-induced leaves and induced samples were sampled at the same time, and RNA was...

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Abstract

Haynaldiavillosa serine / Hv-S / TPK, its coded protein are disclosed. CDNA sequence of Hv-S / TPK is SEQ ID NO.1, its coded amino acid sequence is SEQ ID NO.2. It has better powdery mildew resistance.

Description

1. Technical field: [0001] The invention discloses a serine / threonine kinase gene (Hv-S / TPK) from wheat tufts, which belongs to the field of genetic engineering. The gene can be transferred into susceptible wheat to improve the powdery mildew resistance of wheat. 2. Technical background: [0002] Wheat (Triticum asetivum L.) is the most widely distributed and planted food crop in the world. In my country, the planting area and output of wheat are next to rice, ranking second. Wheat has a long growth period and is susceptible to many biotic and abiotic stresses during its growth. Wheat powdery mildew caused by the obligate parasitic fungus Blumeria graminis f.sp.tritici is the second largest disease of wheat in my country. hazards. The most economical and effective way to control powdery mildew is to use disease-resistant varieties. However, due to the rapid change of pathogenic species, most of the existing varieties in my country have gradually lost their resistance to po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10
Inventor 曹爱忠王秀娥邢莉萍王海燕王苏玲周波陈佩度
Owner NANJING AGRICULTURAL UNIVERSITY
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