Preparation process for semiconductor nanocrystal using Raman signal to marking organism

Abstract

The invention relates to nano material preparation and application in the biology marker, which is characterized by the following: synthesizing different dimensions of nanocrystalline in the anhydrous alcohol; dissolving the perchlorateto and metallic reducing material in the anhydrous alcohol separately; choosing the prepared solution as mother liquid through centrifugation; preparing semiconductor nanocrystalline of Lyman signal within organic ligand; performing biology marker through butt-joint of static electricity and biological molecule. The invention generates resonance action of plasma, which executes the unknown molecule detection through resonance multi-stage Lyman signal of fingerprint feature semiconductor nanocrystalline.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and relates to the preparation of nanomaterials and the application of nanomaterials in biomarkers. Background technique [0002] The assembly of inorganic nanoparticles and biomolecules, and the use of the physical properties of inorganic nanoparticles to implement labeling of biomolecules have become international research frontiers. At present, the physical properties of inorganic nanomaterials used mainly include the plasmonic absorption and coloration of metals, the photoluminescent properties of semiconductor nanocrystals, and the magnetic properties of magnetic nanoparticles. These methods have played an important role in labeling and detecting the properties and activities of biomacromolecules. At present, many research groups in the world are committed to using inorganic fluorescent semiconductor nanocrystal labeling methods to replace organic molecular fluorescent labels....

AI Technical Summary

Problems solved by technology

However, there are also problems with fluorescent labeling of biomolecules by inorganic semiconductor nanocrystals. First, nanocrystals with fluorescent properties generally contain Cd, Pd, HgII-VI semiconductor nanocrystals, etc. These materials are generally environmental unfriendly, harmful to the organism itself

Second, when these materials are coupled with biomolecules, problems such as the phase transfer of nanocrystals and the docking of nanocrystals and biomolecules are involved. These processes are tedious and complicated, and fluorescent nanocrystals that can be applied to biomarkers can be obtained. Crystallization is not easy, which limits the application of inorganic nanocrystals

At present, the bioluminescent labeling method using dyes and quantum dots has shortcomings such as half-peak width, large background interference, and unfriendly environment. A New Method for Labeling Unknown Proteins or Nucleic Acids