Method for preparing semiconductor nano crystal of bio marker utilizing Raman signal

Abstract

This invention belongs to nano-materials and biotechnology fields, involving the preparation of nano-materials, nano-materials in the application of biomarkers. First synthesized in the ethanol the nanocrystals of different sizes, and secondly, reduced perchlorate material and metal were dissolved in ethanol, the solution will be prepared as a mother liquor by centrifugal separation, by adding organic allocation preparation of such a Raman signals in the semiconductor nanocrystals, and through electrostatic and biological molecular docking, the implementation of the markers of biological molecules. This invention produced plasma resonance, allowing semiconductor nanocrystals Resonance Raman signal enhancement. Because of the nanocrystalline metal surface with a group of organic molecules strong coordination role, the organisms can be stable, sound marker, and the further implementation of the use of fingerprint characteristics of semiconductor nanocrystals resonance Raman signal of multi-stage unknown molecular testing.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and relates to the preparation of nanomaterials and the application of nanomaterials in biomarkers. Background technique [0002] The assembly of inorganic nanoparticles and biomolecules, and the use of the physical properties of inorganic nanoparticles to implement labeling of biomolecules have become international research frontiers. At present, the physical properties of inorganic nanomaterials used mainly include the plasmonic absorption and coloration of metals, the photoluminescent properties of semiconductor nanocrystals, and the magnetic properties of magnetic nanoparticles. These methods have played an important role in labeling and detecting the properties and activities of biomacromolecules. At present, many research groups in the world are committed to using inorganic fluorescent semiconductor nanocrystal labeling methods to replace organic molecular fluorescent labels....

AI Technical Summary

Problems solved by technology

However, there are also problems with fluorescent labeling of biomolecules by inorganic semiconductor nanocrystals. First, nanocrystals with fluorescent properties generally contain Cd, Pd, HgII-VI semiconductor nanocrystals, etc. These materials are generally environmental unfriendly, harmful to the organism itself

Second, when these materials are coupled with biomolecules, problems such as the phase transfer of nanocrystals and the docking of nanocrystals and biomolecules are involved. These processes are tedious and complicated, and fluorescent nanocrystals that can be applied to biomarkers can be obtained. Crystallization is not easy, which limits the application of inorganic nanocrystals

[0003] At present, the bioluminescent labeling method using dyes and quantum dots has shortcomings such as half-peak width, large background interference, and unfriendly environment. A New Method for Labeling Unknown Proteins or Nucleic Acids