The invention discloses an extrusion-type anti-skid hand-held grid-counting surface sampling culture dish. The culture dish comprises a dish bottom and a dish cover, wherein a second buckle and a first buckle which are of a stable chimeric structure are arranged on the dish bottom, when under stress, the chimeric structure can loosen to cause relative movement between a mobile dish bottom side wall and a first dish bottom side wall, under cooperation between first hand grabs and second hand grabs, the dish bottom and the dish cover can be unbuckled or buckled more conveniently, when multiple culture dishes are placed in a stacked mode, by means of first bulges and second bulges, relative movement between the two adjacent culture dishes can be limited, and by means of grid lines, a bacterial colony counting process can be simplified.