The invention relates to an application of Lycojaponicumin B in preparation of a human fungus resisting medicine, belonging to the field of medicines. The Lycojaponicumin B has strong effects of inhibiting trichophyton rubrum, Microsporum lanosum or Trichophyton tonsurans, therefore, the Lycojaponicumin B can be used as a compound with a human fungus resisting function, and is expected to be applied in the preparation of the related medicines. The application of the Lycojaponicumin B in preparation of the human fungus resisting medicine, disclosed by the invention, is disclosed for the first time. The skeleton type of the Lycojaponicumin B belongs to a brand-new skeleton type, and the human fungus inhibiting activity of the Lycojaponicumin B is unexpectedly strong, so that no possibility for providing any inspiration by other compounds exists. The Lycojaponicumin B has outstanding substantial characteristics, meanwhile, has a significant progress in prevention and treatment of human fungal infection.