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Cuvette for flow-type particle analyzer

a flow-type particle analyzer and cuvette technology, applied in the field of fluid stream optical analysis, can solve the problems of time-consuming and difficult, and achieve the effects of facilitating use with excitation and detection optics, minimizing vibration of the flow cell or cuvette, and facilitating positioning the cuv

Active Publication Date: 2012-07-31
BECTON DICKINSON & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a cuvette for a flow-type particle analyzer that allows for easy sterilization and replacement of the flow channel without realigning the optics. The cuvette includes a removable flow tube containing the flow channel, which can be easily replaced without disturbing the alignment of the optics. The flow tube is optically transparent, allowing for excitation and detection of particles in the sample. The flow cell also includes a droplet generator for inducing droplets in the sample stream. The droplets are then analyzed in a detection region in the flow channel. The invention also provides a sorting flow cytometer that uses a droplet generator to induce droplets in the sample stream. The invention provides a convenient and stable way to analyze particles in a flow-based system without interfering with the stability of the optics.

Problems solved by technology

Replacement of the components of the fluidic path typically requires realignment of the excitation and detection optics, which is both time-consuming and difficult.

Method used

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  • Cuvette for flow-type particle analyzer
  • Cuvette for flow-type particle analyzer
  • Cuvette for flow-type particle analyzer

Examples

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example 1

Cuvette for use in a Sorting Flow Cytometer

[0054]A flow cell and cuvette essentially as shown in FIG. 5 and described, above, was constructed using standard optical components commercially available for use in the telecommunication and photonics industries.

[0055]A fiber optic ferrule with a rectangular core 127 μm×254 μm was used the flow tube. A fiber optic collimator sleeve designed for use with the ferrule was used as the sleeve tube. The ferrule fitted into the collimator sleeve such that the gap between the ferrule and the collimator sleeve was less than 5 μm. The gap was filled with index-matching fluid using capillary effect. The index-matching fluid also functioned as a lubricant to facilitate the insertion and removal of the ferrule into the sleeve.

[0056]The cuvette block was constructed from borosilicate glass tubing having a rectangular cross-section. Plastic covers on the top and bottom, each having a central hole sized to fit the sleeve tube, were glued to each end of t...

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Abstract

The present invention provides an optical cuvette for use in a flow-type particle analyzer, wherein the cuvette includes a removable flow tube containing a flow channel oriented coaxially.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to the field of optical analysis of fluid streams and, in particular, to flow-type particle analyzers, such as flow cytometers, and particle sorting systems.[0003]2. Description of Related Art[0004]Flow-type particle analyzers, such as flow cytometers, are well known analytical tools that enable the characterization of particles in a fluid stream on the basis of optical parameters such as light scatter and fluorescence. In a flow cytometer, particles such as molecules, analyte-bound beads, or individual cells in a fluid suspension are passed by one or more detectors in which the particles are exposed to an excitation light, typically from one or more lasers, and the light scattering and fluorescence properties of the particles are measured. Each particle, or subcomponents thereof, may be labeled with a multiplicity of spectrally distinct fluorescent dyes. Typically, detection or characteriz...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N1/10
CPCG01N15/1434G01N21/05G01N2015/149G01N2021/0346G01N15/149
Inventor CHEN, YONG QIN
Owner BECTON DICKINSON & CO
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