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Composition for enhancing immunity or preventing or treating neurodegenerative diseases by using goji berries, mulberries and jujubes

Inactive Publication Date: 2020-12-10
COSMAX NS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a composition that uses a mixture extract of goji berries, mulberries, and jujubes or an extract mixture thereof as an active ingredient. This composition has a synergistic effect on enhancing the immune system and preventing, treating, or ameliorating neurodegenerative diseases without causing cytotoxicity. The weight ratio of goji berry to mulberry to jujube is important. When the ratio is between 1 to 3:1.5 to 3.5, the composition has a synergistic effect on both immune enhancement and memory improvement. The mixture extract or the extract mixture can be used as a pharmaceutical or health functional food.

Problems solved by technology

Researches to show the immune enhancement efficacy through these macrophages or to prevent, treat or ameliorate neurodegenerative diseases are actively conducted, but there are no studies on goji berries, mulberries and jujubes as a raw material in relation to the symptoms.

Method used

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  • Composition for enhancing immunity or preventing or treating neurodegenerative diseases by using goji berries, mulberries and jujubes
  • Composition for enhancing immunity or preventing or treating neurodegenerative diseases by using goji berries, mulberries and jujubes
  • Composition for enhancing immunity or preventing or treating neurodegenerative diseases by using goji berries, mulberries and jujubes

Examples

Experimental program
Comparison scheme
Effect test

experimental example 1

city

[0091]Cell viability was measured in order to evaluate the cytotoxicity of the composition prepared in Examples 1 and 2 and Comparative Examples 1 to 3.

[0092]First, mouse macrophage cell line RAW264.7 cells were cultured at a density of 70-80% in a 100 mm cell culture dish using RPMI 1640 medium containing penicillin (100 IU / ml) and streptomycin (100 μg / ml) and 10% FBS.

[0093]Then, it was analyzed using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. The cultured mouse macrophage cell line RAW264.7 cells were adjusted to a concentration of 1×106 cell / ml using RPMI 1640 medium, and then inoculated into a 96 well plate and precultured at 5% CO2 and 37° C. for 18 hours. Thereafter, the medium was removed, and culture was performed in the medium treated with the test material at a concentration shown in the figures. After 24 hours, 10 μl MTT solution (stock concentration: 5 mg / ml) was added to induce an additional reaction for 3 hours. 100 μl DMSO was additi...

experimental example 2

phage-Derived TNF-α Expression

[0095]In order to compare the immune enhancement efficacy of the compositions prepared in Examples 1 and 2 and Comparative Examples 1 to 3, the TNF-α expression was measured.

[0096]First, the cultured mouse macrophage cell line RAW264.7 cells in Experimental Example 1 were adjusted to a concentration of 1×106 cell / ml using RPMI 1640 medium, and then inoculated into a 96 well plate and precultured at 5% CO2 and 37° C. for 18 hours. Thereafter, the medium was removed, and culture was performed in the medium treated with the test material at a concentration shown in the figures. After 24 hours, the supernatant was transferred to another 96 well plate by 100 μl, and measured the macrophage-derived TNF-α expression by using an enzyme-linked immunosorbent assay (ELISA) kit of R&D systems according to the manufacturer's instructions, and the results are shown in FIG. 2.

[0097]As shown in FIG. 2, the macrophage-derived TNF-α expression in the case of treating wit...

experimental example 3

f mBDNF to Hippocampal Total BDNF

[0099]In order to compare the efficacy of preventing, treating or ameliorating neurodegenerative diseases of the compositions prepared in Examples 1 and 2 and Comparative Examples 1 to 3, total BDNF expression and mature BDNF expression were measured.

[0100]First, the cultured mouse hippocampal cell line HT22 cells in Experimental Example 1 were adjusted to a concentration of 1×104 cell / ml using DMEM medium, and then inoculated into a 96 well plate and preincubated at 5% CO2 and 37° C. for 18 hours. Thereafter, the medium was removed, and culture was performed in the medium treated with the test material at a concentration shown in the figures. After 24 hours, the supernatant was transferred to another 96 well plate by 100 μl, and measured a concentration of total BDNF and a concentration of mature BDNF by using an enzyme-linked immunosorbent assay (ELISA) kit of R&D systems and calculated a ratio of mature BDNF to total BDNF, and the results are show...

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Abstract

The present invention relates to a composition for enhancing immunity or a composition for preventing or ameliorating neurodegenerative diseases, both of which contain, as an active ingredient, a mixture extract of goji berries, mulberries and jujubes or an extract mixture thereof.

Description

BACKGROUND OF THE INVENTION1. Field of the Invention[0001]The present invention relates to a composition for enhancing immunity or preventing or ameliorating neurodegenerative diseases, which comprises a mixture extract of goji berries, mulberries and jujubes or an extract mixture thereof.2. Description of the Related Art[0002]Macrophage is a cell that regulates immune phenomena by secreting various materials in the process of phagocytosis and removal of bacteria or foreign substances and plays a pivotal role in the immune response to antigens, and involves in antigen presentation and non-specific immune response of lymphocytes, and has direct injury activity against tumor cells. In addition, it is known substances (LPS or natural products) responding to toll-like receptors (TLR) activate macrophages to produce cytokines such as IL-1, IL-6, IL-10, 1L-12 and TNF-α which can regulate secondary immune responses such as defense against microbial infection, activation of macrophages for ...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61K36/605A61K36/725A23L33/105
CPCA61K36/605A61K2236/51A61K36/815A61K36/725A23L33/105A23V2002/00A23V2200/322A23V2200/324A61P25/00A61P37/00A61K2236/00A61K2300/00
Inventor BAEK, KWANG SOOKIM, HYUNJICHOI, SU YOUNGKIM, JIN HAKSHIM, JAE SEOKPARK, YOUNG MINKIM, SANG WOOYOON, MANN-SEOKLEE, YOON JONG
Owner COSMAX NS INC
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