Stability of silage inoculants and methods for improving aerobic stability of silage
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example 1
[0031]Silage was made with fresh cut sugar cane from plants that were approximately 12 months old. The sugar cane was manually harvested and chopped using a laboratory-type chopper (Pinheiro, model: PP-47) to an approximate length of 30 mm. 3 kg of the chopped material was mixed with the inoculants and conditioned in PVC plastic buckets (mini-silos, 10 cm in diameter and 60 cm in length), which were sealed with tight lids containing Bunsen valves for gas release. The material in the silo was compacted to a density of approximately 630±19.9 kg m-3. The mini-silos were stored at room temperature and analyzed after 61 days of storage, and three replicates were prepared for each silo.
[0032]Silage was produced using the Lactobacillus plantarum SIL 34 (L. plantarum are commonly used as silage inoculant) and the Lactobacillus hilgardii strains SIL 51 (CNCM I-4784 filed on Jun. 26, 2013) and SIL 52 (CNCM I-4785 filed on Jun. 26, 2013) as inoculants. The Lactobacillus plantarum and the Lacto...
example 2
Aerobic Stability of Corn Silages
[0048]Corn silage was produced in micro-silos as described in Example 1 using the Lactobacillus buchneri, NCIMB 40788 (U.S. Pat. No. 6,326,037 to Mann et al.), the Lactobacillus plantarum SIL 34 and the Lactobacillus hilgardii strains SIL 51 (CNCM I-4784 filed on Jun. 26, 2013) and SIL52 (CNCM I-4785 filed on Jun. 26, 2013) as inoculants. Silage without any inoculants was used as a control. The inoculants were cultured as described in Example 1.
[0049]After 90 d of ensiling, the mini-silos were opened, and samples of approximately 3 kg were removed from each mini-silo and placed in 5 kg plastic buckets to assess the aerobic stability. A data logger was inserted into the silage mass, at a depth of 10 cm, for 7 days. The ambient temperature was measured using a data logger located close to the buckets. The data on aerobic stability of silages are shown in Table 4
TABLE 4Aerobic stability of corn silages with inoculants.MaximumTime to reachAerobictemperat...
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