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Glycated cd59 peptides, their preparation, and uses thereof

a technology of cd59 and peptides, which is applied in the field of glycated cd59 peptides, their preparation, can solve the problems of achieve the effects of facilitating the unregulated activation of complement and excessive and accelerated deposition of mac, stimulating proliferation of fibroblasts, and increasing the synthesis of extracellular matrix proteins

Inactive Publication Date: 2015-11-05
PRESIDENT & FELLOWS OF HARVARD COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent is about a new discovery of a molecule called CD59, which is involved in regulating the body's response to complement, a part of the immune system. CD59 helps prevent the excessive buildup of a harmful substance called membrane attack complex (MAC) in blood vessels and kidneys, which can contribute to the development of complications in people with diabetes. The patent also describes a new antibody that specifically targets the glycated form of CD59, which is associated with disease-related changes in the body. This antibody can be used to diagnose and monitor the progression and regression of diabetes and pre-diabetes, as well as other diseases. The patent also mentions that CD59 is present in urine and saliva, making it a potential biomarker for these conditions.

Problems solved by technology

Thus, glycation of CD59 disrupts its regulatory function and effectively enables the unregulated activation of complement and excessive and accelerated deposition of MAC.

Method used

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  • Glycated cd59 peptides, their preparation, and uses thereof
  • Glycated cd59 peptides, their preparation, and uses thereof
  • Glycated cd59 peptides, their preparation, and uses thereof

Examples

Experimental program
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Effect test

example 1

General Peptide Coupling Procedure

[0205]Nα-Ac[Lys41(Nε-1-deoxyfructosyl)]hCD59(37-50)-OH polypeptide (SEQ ID NO:3) was manually synthesized via stepwise solid-phase polypeptide synthesis from 155 mg of Fmoc-Cys(Trt)-Rink resin starting material (0.41 meq / g, 0.0633 mmol) using PyBOP, HOBt, and DIEA coupling reagents in 5 / 5 / 10-fold molar excess. Successive Nα-Fmoc protected amino acids were added in a 5-fold molar excess and stirred 2-3 hours at ambient temperature in the dark. Side-chain protecting groups of the Nα-Fmoc protected amino acids included: Asn(Trt), Asp(OtBu), Glu(OtBu), His(Nim-Trt), Lys(Nε-Boc) and Trp(Nin-Boc). Synthesis of derivatives of the above polypeptide follow a similar procedure.

[0206]The Amadori modification: The Amadori modification of lysine proved successful only when fully protected Amadori building blocks of lysine were used. Thus, the fully protected Amadori building block (1), Nα-Fmoc-Lys[Nε-(2,3:4,5-di-O-isopropylidene-1-deoxyfructosyl, Nε-Boc)]-OH, wa...

example 2

Synthesis of fully protected Nα-Fmoc-Lys[Nε-(2,3:4,5-di-O-isopropylidene-1-deoxyfructosyl, Nε-Boc)]-OH (1)

[0209]

Step 1: Synthesis of Nα-Fmoc-Lys[Nε-(2,3:4,5-di-O-isopropylidene-1-deoxyfructosyl)]-OH (2)

[0210]A fresh solution of NaCNBH3 (600 mg, 9.5 mmol) in 5 mL H2O / THF (1:1, v / v) was added to a stirred solution of Nα-Fmoc-Lys-OH (1.4 g, 3.8 mmol) and 2,3:4,5-di-O-isopropylidene-aldehydo-β-D-arabino-hexos-2-ulo-2,6-pyranose (2.45 g, 9.5 mmol) in 15 mL H2O / THF (1:1, v / v) under N2 at 50° C. After 4 h the solvent was removed under reduced pressure and the crude product (RfA for the product=0.8, RfA for the starting material=0.45; UV & ninhydrin positive) was purified (RP-FC) using a linear gradient 30-50% B in A over 20 minutes. The intended product eluted at 37% B in A. The pooled fractions were lyophilized. Pure (2) was obtained as a white solid (500 mg, 22%). LC-ESI-MS m / z=611 ([M+H]+), calcd MW=610 at Rt=3.06 minutes using a linear gradient of 30-80% B in A over 5 min; at Rt=3 min ...

example 3

Synthesis of Nα-Fmoc-Lys[Nε-(1-deoxyfructosyl)]-OH (3)

[0212]

[0213]FmocLys-OH (1 g, 2.71 mmol) and D-glucose (1.22 g, 6.77 mmol, 2.5 eq) were suspended in 40 mL of dry DMF under an atmosphere of N2. The reaction was heated to 110° C. for 10 minutes then poured into an ice bath. After a few minutes the Fmoc-Lys-OH dissolved in DMF as a yellow solution. The reaction had to be stopped before the solution was allowed to rapidly turned brown, which indicates the occurrence of diglycated product. The DMF was evaporated under vacuum and the crude residue was purified by RP-FC. TLC analysis with DCM / MeOH (1:4) eluant was visualized with ninhydrin. Pure (3) was obtained as a white solid (390 mg, 67%). LC-ESI-MS calcd: MW=530. found m / z=531 ([M+H]+), Rt=2.13 min using a linear gradient of 30-50% B in A over min 5 min; analytical RP-HPLC was employed using a linear gradient of 40-80% B in A over 3 min; R, =1.45 min. 1H NMR (CDCl3, 400 MHz,): δ 7.74 (d, 2H, J3,4=J5,6=7.4 Hz, fluorenyl 4-H and 5-...

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Abstract

The present invention provides glycated Amadori products of the CD59 peptide and fragments thereof to be used as tools and among methods for the diagnosis and prognosis of pre-diabetes and diabetes. Certain aspects of the invention include glycated Amadori products of CD59 and fragments thereof to be used for the generation of antibodies and antibody fragments. Still other aspects of the invention include methodologies for the preparation of glycated Amadori products of CD59, fragments thereof, the inventive antibodies, and antibody fragments.

Description

RELATED APPLICATIONS[0001]The present application claims priority under 35 U.S.C. §119(e) to U.S. provisional application, U.S. Ser. No. 61 / 377,060, filed Aug. 25, 2010, which is incorporated herein by reference.BACKGROUND OF THE INVENTION[0002]Diabetes mellitus (diabetes) is a leading cause of morbidity and mortality in the adult population. This is primarily because diabetic patients tend to develop vascular complications that involve the kidneys (diabetic nephropathy), the retina (diabetic retinopathy), as well as large and small blood vessels in other organs (macro- and micro-vascular disease) including nerves (diabetic neuropathy). It is well established that the vascular complications of diabetes are caused by elevated blood glucose levels over long periods of time. Elevated blood glucose levels affect proteins by a nonenzymatic process known as glycation. Glycation, the non-enzymatic attachment of glucose to proteins, is considered a major post-translational modification caus...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K16/28G01N33/68
CPCC07K16/2896G01N33/6872C07K2317/54G01N2800/042C07K2317/34G01N2333/70596G01N2440/38C07K2317/55C07K14/70596G01N33/6842A61P3/00A61P3/10
Inventor HALPERIN, JOSE A.CHOREV, MICHAEL
Owner PRESIDENT & FELLOWS OF HARVARD COLLEGE
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