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Acellular soft tissue-derived matrices and methods for preparing same

a soft tissue and matrice technology, applied in the field of matrices made from decellularized soft tissues, can solve the problems of tissue rejection, significant risks of biological material transfer from one individual to another,

Inactive Publication Date: 2015-02-05
MUSCULOSKELETAL TRANSPLANT FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Embodiments of this patent relate to a method for creating a soft tissue-derived matrix that can be used in plastic surgery procedures. The method involves isolating a desired soft tissue, decellularizing the tissue by soaking it in a hypertonic solution and a surfactant, and then removing lipids from it. The resulting matrix can be in various forms such as particulate, sheet, or injectable form. The method also involves adding factors to promote tissue growth and differentiation, such as growth factors. Overall, this technology provides a way to create a safe and effective soft tissue-derived matrix for use in plastic surgery procedures.

Problems solved by technology

The transfer of biological material from one individual to another can raise significant risks.
One such risk is tissue rejection, which can occur even in cases where there is a good histocompatibility match.

Method used

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  • Acellular soft tissue-derived matrices and methods for preparing same
  • Acellular soft tissue-derived matrices and methods for preparing same

Examples

Experimental program
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Effect test

example 1

Decellularization with Sodium Deoxycholate

[0620]Adipose tissue is recovered aseptically from a cadaveric donor. Any muscle, fascia, or other tissues attached to the adipose tissue, if present, are cut away. Equal portions of the adipose tissue are measured into empty flasks and washed by agitation in deionized water, at a ratio of about 1000 ml of water to 500 gm of tissue. After a period of time sufficient to remove blood and loose tissue from the adipose tissue, the water is drained from the flask, while collecting the adipose tissue in a 212 μm to 300 μm sieve. After the water is drained, the adipose tissue is returned to the flask.

[0621]Following the washing step, the adipose tissue is soaked with mechanical agitation in a 4% solution of sodium deoxycholate, at a ratio of about 1000 mL deoxycholate solution to 500 gm of adipose tissue. After a period of time sufficient to disrupt and remove the adipocytes and other cells from the adipose tissue, the deoxycholate solution is drai...

example 2

Decellularization with a Hypertonic Solution

[0624]A sample of adipose tissue is measured into a flask and a hypertonic solution (e.g., 1M NaCl) is added in a 2:1 ratio. The mixture is agitated for at least 12 hours at an ambient temperature. After agitation, the hypertonic solution is decanted, and the adipose tissue is captured in a 212 μm to 300 μm sieve. The recovered adipose tissue is returned to the flask, and soaked in an 0.1% surfactant solution with agitation for at least 12 hours at ambient temperature.

[0625]Following the last water rinse, the adipose tissue is soaked in a sterilizing solution, with agitation. One suitable sterilizing solution would be 0.5% to 1.0% peracetic acid in a mixture of water, ethanol, and propylene glycol. One or more additional soaks in sterilizing solution may be needed to adequately sterilize the adipose tissue. After the final sterilizing soak, the adipose tissue is rinsed repeatedly in deionized water to remove any traces of sterilizing solut...

example 3

Removal of Lipids from an Adipose Tissue

[0627]A sample of adipose tissue, which may be decellularized or untreated, is collected and a size reduction, such as grinding or mincing, is performed. At a temperature of ambient or greater, the adipose tissue is placed in a conical tube or beaker, and homogenized. The homogenized tissue is then centrifuged to separate lipid and water layers from the tissue, taking care not to lose the floating tissue layer.

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Abstract

An acellular soft tissue-derived matrix includes a collagenous tissue that has been delipidated and decellularized. Adipose tissue is among the soft tissues suitable for manufacturing an acellular soft tissue-derived matrix. Exogenous tissuegenic cells and other biologically-active factors may be added to the acellular matrix. The acellular matrix may be provided as particles, a slurry, a paste, a gel, or in some other form. The acellular matrix may be provided as a three-dimensional scaffold that has been reconstituted from particles of the three-dimensional tissue. The three-dimensional scaffold may have the shape of an anatomical feature and serve as a template for tissue repair or replacement. A method of making an acellular soft tissue-derived matrix includes steps of removing lipid from the soft tissue by solvent extraction and chemical decellularization of the soft tissue.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]The present application claims the benefit of U.S. Provisional Patent Application No. 61 / 860,043, filed on Jul. 30, 2014, the disclosure of which is incorporated by reference herein.FIELD OF THE INVENTION[0002]The present invention relates generally to matrices made from decellularized soft tissues, including adipose, dermis, fascia, muscle, pericardium, and other connective or membranous tissues, and in particular, to such matrices as are suitable for implantation into a living body in plastic surgery procedures, including reconstructive or cosmetic surgery procedures.BACKGROUND OF THE INVENTION[0003]Techniques for restoring structure and function to damaged tissue are used routinely in the field of plastic surgery, including reconstructive and cosmetic surgery. Tissue transplantation is one way of restoring structure and function by replacing or rebuilding the damaged tissue. The transfer of biological material from one individual to ano...

Claims

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Application Information

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IPC IPC(8): A61K35/12
CPCA61K35/35A61L27/3604A61L27/3683A61L2430/40
Inventor HUANG, YEN-CHENIVERY, ASIACHOI, BRYANSCHILLING, BENJAMINNGO, MANH-DAN
Owner MUSCULOSKELETAL TRANSPLANT FOUND INC
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