Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA sequencing using multiple metal layer structure with organic coatings forming transient bonding to DNA bases

Inactive Publication Date: 2012-08-02
IBM CORP
View PDF3 Cites 58 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a nanodevice that can control molecules using electricity. The device includes a reservoir filled with ionic fluid and a membrane separating the reservoir. The membrane has electrode layers separated by insulating layers in which the electrode layers have an organic coating. The membrane also has a nanopore through which molecules can pass. When a voltage is applied to the electrodes, a tunneling current is generated by the base of the molecule in the nanopore, which is measured as a current signature to distinguish the base. The patent also describes a method for controlling molecules in the nanodevice. Overall, the nanodevice provides a way to manipulate molecules with precision using electricity.

Problems solved by technology

Two issues in these applications of nanopores are the control of the translocation of DNA through the nanopore and differentiating DNA bases.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA sequencing using multiple metal layer structure with organic coatings forming transient bonding to DNA bases
  • DNA sequencing using multiple metal layer structure with organic coatings forming transient bonding to DNA bases
  • DNA sequencing using multiple metal layer structure with organic coatings forming transient bonding to DNA bases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019]Exemplary embodiments are based on an organic-coated nanopore through a stack of repetitive insulating / conducting / insulating / conducting / insulating / . . . layers. Exemplary embodiments are configured to leverage the transient bonding between the organic coating and the DNA bases to control the motion of the DNA, and then utilize the tunneling current through the DNA base for differentiating the DNA base while the DNA base is transiently bonded to the organic coating. This is called a transient bond because an external electric field can by applied to move the DNA molecule and thus break the transient bond to the DNA base.

[0020]Exemplary embodiments may use a nanopore through a stack of repetitive insulating / conducting / insulating / conducting / insulating / . . . layers where all conductive surfaces (of the conducting / electrode layers) at the inner-surface of the nanopore are coated with an organic coating. This organic coating can transiently bond to individual DNA bases and thus th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
voltageaaaaaaaaaa
tunneling currentaaaaaaaaaa
currentaaaaaaaaaa
Login to View More

Abstract

A nanodevice is provided. A reservoir is filled with an ionic fluid. A membrane separates the reservoir, and the membrane includes electrode layers separated by insulating layers in which the electrode layers have an organic coating. A nanopore is formed through the membrane, and the organic coating on the electrode layers forms transient bonds to a base of a molecule in the nanopore. When a first voltage is applied to the electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels through the transient bonds formed to the base to be measured as a current signature for distinguishing the base.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The application is based on and incorporates by reference the content of U.S. Provisional Patent Application 61 / 437,101 filed on Jan. 28, 2011, and priority is claimed there from under applicable sections of 35 U.S.C. §119 or 120.BACKGROUND[0002]Exemplary embodiments relate to nanodevices, and more specifically to sequencing using a multiple layer structure in a nanodevice.[0003]Recently, there has been growing interest in applying nanopores as sensors for rapid analysis of biomolecules such as Deoxyribonucleic acid (DNA), Ribonucleic acid (RNA), protein, etc. Special emphasis has been given to applications of nanopores for DNA sequencing, as this technology holds the promise to reduce the cost of sequencing below $1000 / human genome. Two issues in these applications of nanopores are the control of the translocation of DNA through the nanopore and differentiating DNA bases.[0004]Nanopore sequencing is a method for determining the order in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): G01N27/447G01N27/453
CPCG01N33/48721C12Q1/6869C12Q2563/116C12Q2563/157C12Q2565/607C12Q2565/631
Inventor AFZALI-ARDAKANI, ALIHARRER, STEFANLUAN, BINQUANMARTYNA, GLENN J.PENG, HONGBOROSSNAGEL, STEPHEN M.STOLOVITZKY, GUSTAVO A.WAGGONER, PHILIP S.WALKER, GEORGE F.
Owner IBM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com