Pcv2 mycoplasma hyopneumoniae immunogenic compositions and methods of producing such compositions

Abstract

Multivalent combination vaccines are provided which include an immunological agent effective for reducing the incidence of or lessening the severity of M. hyo infection, preferably M. hyo bacterin, or an immunogenic composition comprising M. hyo bacterin, and at least one immunogenic active component of another disease-causing organism in swine, preferably PCV2 wherein the preferred PCV2 antigen for such a multivalent vaccine is PCV2 ORF 2 protein.

Description

RELATED APPLICATIONS[0001]This application claims the priority benefit of U.S. provisional application Ser. No. 61 / 023,086, filed on Jan. 23, 2008 and U.S. provisional Ser. No. 61 / 025,293, filed on Jan. 31, 2008. The teachings and content of both of these applications are hereby incorporated by reference herein.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]In one aspect of the present invention, an immunogenic composition effective for inducing an immune response against infection by Mycoplasma hyopneumoniae (M. hyo) is provided. More particularly, the present invention is concerned with an immunogenic composition effective for inducing, eliciting, or providing an immune response that protects an animal receiving such a composition and reduces the incidence of, or lessens the severity of, the clinical symptoms associated with M. hyo infection. Still more particularly, the present invention is concerned with an immunogenic composition that includes therein cell-base...

AI Technical Summary

Benefits of technology

[0018]As used herein, a “protective immune response” refers to a reduced incidence of or reduced severity of clinical, pathological, or histopathological signs of infection from M. hyo or PCV2 infection up to and including the complete prevention of such signs.

[0048]Injection timing is flexible. Compositions as described herein can be used as early as three weeks of age through the time when pigs leave the nursery with the objective of vaccinating at least 2 weeks prior to exposure to Mycoplasma hyopneumoniae. The composition according to the invention may be applied in any conventional manner including intradermally, intratracheally, or intravaginally. The composition preferably may be applied intramuscularly or intranasally. In an animal body, it can prove advantageous to apply the pharmaceutical compositions as described above via an intravenous injection or by direct injection into target tissues. For systemic application, the intravenous, intravascular, intramuscular, intranasal, intra-arterial, intraperitoneal, oral, or intrathecal routes are preferred. A more local application can be effected subcutaneously, intradermally, intracutaneously, intracardially, intralobally, intramedullarly, intrapulmonarily or directly in or near the tissue to be treated (connective-, bone-, muscle-, nerve-, epithelial tissue). Depending on the desired duration and effectiveness of the treatment, the compositions according to the invention may be administered once or several times, also intermittently, for instance on a daily basis for several days, weeks or months, and in different dosages. However, as detailed herein, the combination of M. hyo bacterin and PCV2 ORF 2 provides an immunogenic composition that provides effective immunity and / or reduces the severity of or incidence of clinical signs associated with M. hyo and / or PCV2 infection in pigs receiving such a composition after just one dose.

Problems solved by technology

However, these procedures have a disadvantage in that the extraction procedures are both costly and time-consuming.

Additionally, the amount of ORF2 recovered from the cells is not very high; consequently, a large number of cells need to be infected by a large number of viral vectors in order to obtain sufficient quantities of the recombinant expressed protein for use in vaccines and the like.