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Method, kit, and apparatus for cancer detection using urinary tumor markers

a cancer and urine technology, applied in the field of cancer detection methods, kits, and apparatuses, can solve the problems of excessive examination and therapy, and examination does not contribute to effective reduction of mortality

Active Publication Date: 2022-08-16
NAGOYA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0036]The present invention provides a method, an apparatus, and a kit for easily and inexpensively detecting a neuroblastoma with minimal invasion. In particular, the method, apparatus, and kit can determine whether the neuroblastoma is stage 4S neuroblastoma, which has a good prognosis. This can avoid unnecessary therapy and examination and can select an appropriate therapy for a neuroblastoma other than stage 4S neuroblastoma. The examination performed is an examination using urine. This extremely simplifies the collection or sampling technique in clinical site and significantly increases the convenience of medical care workers. The present invention is therefore useful in the fields typically of diagnosis, examination, therapy evaluation, and drug development for neuroblastoma.

Problems solved by technology

Unfortunately, questions remain as to the efficacy of the examination (for example, Japanese Unexamined Patent Application Publication No.
Specifically, the examination does not contribute to effectively decreased mortality; and excessive examination and therapy becomes a problem, because some neuroblastoma have a good prognosis, and may spontaneously regress.

Method used

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  • Method, kit, and apparatus for cancer detection using urinary tumor markers
  • Method, kit, and apparatus for cancer detection using urinary tumor markers
  • Method, kit, and apparatus for cancer detection using urinary tumor markers

Examples

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Effect test

example 1

sive Analysis of Neuroblastoma-Related Urinary Metabolites

[0155]To identify urinary tumor markers specific to neuroblastoma, a global analysis of urinary metabolites was performed on 58 healthy children and 7 neuroblastoma-affected children before therapy, in Nagoya University Graduate School of Medicine, Surgery, Pediatric Surgery Laboratory.

[0156]Specifically, metabolites in urine of neuroblastoma-affected children and in urine of healthy children of the same age were comprehensively analyzed using a liquid chromatograph-mass spectrometer (LC / MS) to specify metabolites that significantly increase or decrease in the childhood cancer-affected (neuroblastoma-affected) children as compared with the healthy children, to thereby find urinary tumor markers that can distinguish between healthy children and childhood cancer-affected children by multivariate analysis. As a result, urinary tumor markers that distinguish between the two groups were found (Japanese Patent Application No. 2018-...

example 2

ion of Cancer Evaluation Model Using Group (A) Markers and Group (B) Markers

[0163]On each of the Group (A) markers and the Group (B) markers selected in Example 1, prediction models were made by OPLS-DA on all combinations, and the performance indices (in particular, predictor variables) of the models were determined. In particular, combinations of urinary metabolites involved in different metabolisms were selected as marker candidates.

[0164]Specifically, to distinguish between healthy children and neuroblastoma-affected children, cancer examination models were determined by orthogonal projections to latent structures-discriminant analysis (OPLS-DA) using indices expressed as follows:

[0165]R⁢⁢2⁢Y=1-Σ⁡(Yobs-Ycalc)2Σ⁡(Yobs-Y_)2Q⁢⁢2=1-Σ⁡(Yobs-Ypred)2Σ⁡(Yobs-Y_)2

[0166]In the expressions, Yobs represents the measured value; Ycalc represents the value calculated by OPLS; Ypred represents the predicted value according to cross-validation; and represents the average. The “cross-validation” ...

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Abstract

Provided are a method, an apparatus, and a kit for detecting a neuroblastoma in a subject and / or for monitoring a therapeutic effect on the neuroblastoma, by measuring a urinary tumor marker(s) in a sample from the subject.

Description

RELATED APPLICATION[0001]This application claims the benefit of priority to Japanese Patent Application number 2018-212341, filed Nov. 12, 2018, hereby incorporated by reference in its entirety.TECHNICAL FIELD[0002]The present invention relates to methods, kits, and apparatuses for detecting a neuroblastoma in a subject using urinary tumor markers derived from the subject.BACKGROUND ART[0003]Neuroblastoma is one of childhood cancers and is often diagnosed at 0 to 4 years of age. Neuroblastoma tumor cells produce catecholamine, which is a neurotransmitter, and the catecholamine converts into vanillylmandelic acid (VMA) and homovanillic acid (HVA), and they are excreted into the urine. Neuroblastoma mass screening examination that detects or determines VMA and HVA has been performed on six-month-old infants as subjects, to detect neuroblastoma early. Unfortunately, questions remain as to the efficacy of the examination (for example, Japanese Unexamined Patent Application Publication N...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N33/574G16H50/30G16B40/10G01N30/06G01N30/02
CPCG01N33/57484G01N30/06G01N33/57407G16B40/10G16H50/30G01N2030/027G01N2030/065G01N2030/067G01N2800/56G01N2800/60G01N2800/52G01N33/5011
Inventor UCHIDA, HIROOHINOKI, AKINARISAKAIRI, MINORUABE, MAYUMI
Owner NAGOYA UNIVERSITY
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