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Rice breeding method incorporating high photosynthetic efficiency and presenility resistant property

A high-light-efficiency, rice-based technology, applied in the fields of botanical equipment and methods, introduction of foreign genetic material using vectors, plant genetic improvement, etc., can solve the problems of low photosynthetic efficiency, easy aging, etc. Effects of Genetic Disorders

Inactive Publication Date: 2007-07-11
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is: aiming at the low photosynthetic efficiency and easy senescence commonly existing in rice at present, especially the practical problems that senescence directly affects the yield and quality of rice, we use the existing ZL01138012.8 patent technology to conduct in-depth research, Provide a rice breeding method that combines high light efficiency and anti-aging traits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Transfer of γ-ECS gene into Suhuxiangjaponica

[0023] In 2003, the γ-ECS gene of maize was transferred, and the specific method was as follows: the mature rice seeds of Suhu Xiangjaponica were dehulled, treated with 75% alcohol for 1 min, soaked in 0.1% mercuric chloride solution for 15 min, and then soaked in 0.1% mercuric chloride solution for 15 min. Bacterial water was rinsed 3 times, and inoculated on the induction callus medium with M8 as the basic medium, the type and ratio of hormones (the formula for inducing mature embryo callus is M8+2mg / L2, 4-D1mg / LABA +1mg / L6-BA, 3% sucrose+7% agar) were cultured in the dark at 28°C for 30d. Cut the induced yellow-white dense callus from the scutellum of the seed embryo, immerse it in the bacterial solution of the Agrobacterium strain GV3010 carrying the γ-ECS gene, and soak it for 5 minutes, then co-culture for 48 hours, and then insert it containing 500mg / In the selection medium of L cephalosporin and 50 mg / L...

Embodiment 2

[0030] Example 2 Obtaining F1 of γ-ECS-Suhuxiangjaponica / PEPC Gene Transgenic Rice

[0031] In 2004, the anti-aging material H obtained in Example 1 2 The germplasm is used as the female parent, obtained according to the method disclosed in the ZL01138012.8 patent application by Jiao Demao, Gao Dongying, Li Xia, etc. 4 The rice germplasm stably expressing the plant PEPC gene was used as the male parent, and 112 hybrid F1 seeds were obtained by crossing.

Embodiment 3

[0032] Example 3 F1 screening of high light efficacy and anti-aging properties

[0033] In the winter of 2004, all 112 grains of hybrid F1 materials obtained by Example 2 were planted in Hainan. By identifying the PEPC activity and photosynthetic rate of the leaves at the heading stage, the chlorophyll content and Fv / Fm, 55 rice materials with high light efficiency and anti-aging resistance were selected F 1 Materials, the PEPC activities of the screened materials are all between 260-1099 μmol / mgChl.h, and the photosynthetic rate values ​​are 17.2-29.8 μmol / m 2 .s, the Fv / Fm attenuation is 5%-10%, and the chlorophyll content attenuation is 12-20%, both of which have high photosynthetic efficiency and anti-aging properties, which proves that sexual crosses can be used to obtain a combination of two excellent physiological properties. Material.

[0034] High efficacy characteristics are identified by measuring the C 4 Photosynthetic enzyme PEPC activity and leaf photosyntheti...

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PUM

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Abstract

The invention relates to a rice seeding method which comprises that: leading decay gene into rice seed to obtain the rice system; crossing the rice with C4 plant PEPC gene stable representation with the rice system, to obtain F1 system; checking, selecting the rice with high light effect and anti-decay character; cultivating F1, to obtain two flower systems; checking, selecting the rice pure system with high light effect and anti-decay character.

Description

technical field [0001] The invention relates to the field of molecular physiological breeding, in particular to a rice breeding method that integrates high light efficiency and anti-aging traits by organically combining genetic engineering, conventional breeding and physiological identification. Background technique [0002] The dwarfing of rice varieties and the three-line matching of indica hybrid rice are two important milestones in the history of modern rice breeding, and they have greatly increased rice yield. The average yield of rice in my country has reached 6t / hm 2 , Jiangsu has exceeded 8t / hm 2 , the leaf area index is about 8-10, and it is quite difficult to increase the yield by increasing the leaf area of ​​the population. The light energy utilization rate of the existing high-yield rice varieties is analyzed at 1-1.5%, while the ideal light energy use rate can reach 3-5%. It can be seen that improving the photosynthetic efficiency of rice may be one of the ef...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H3/00A01H5/00C12N15/82
Inventor 李霞周月兰王超孙志伟郭士伟何冰焦德茂
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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