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Microorganism nitrite degradation agent and preparation method thereof

A technology of nitrite degradation and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor resistance to adverse environmental impacts and unstable treatment effects, so as to reduce water pollution and apply The effect of wide range and guaranteed effectiveness

Inactive Publication Date: 2007-07-04
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, single or compound microbial strains are generally used to control water quality at home and abroad, but because microorganisms are greatly affected by the external environment and have poor resistance to adverse environmental impacts, once the system is damaged, it is difficult to recover, so the treatment effect is unstable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Microbial nitrite degradation agent and preparation method, comprising the following steps:

[0036] The first step: Bacillus subtilis is inoculated in liquid culture medium, and it is prepared by the raw material of following percentage by weight: bean cake powder 2.5%, corn flour 4%, starch 2%, molasses 0.5%, potassium dihydrogen phosphate 0.03%, Disodium hydrogen phosphate 0.5%, silicone defoamer 0.1%, water 90.37%, the temperature is 37°C, and the incubation time is 24 hours;

[0037] The second step: inoculate the nitrifying bacteria in the enrichment medium, which is prepared by the following raw materials in weight percentage: 0.5% ammonium sulfate, 1.0% sodium bicarbonate, 0.4% sodium chloride, 0.04% magnesium sulfate heptahydrate, 0.04% magnesium sulfate heptahydrate, Ferrous sulfate water 0.02%, calcium chloride dihydrate 0.08%, glucose 0.08%, water 97.88%; control pH=7.5~8.5, temperature 28~30℃, Vs: 30%~40%, (DO)>2mg· L-1, carry out continuous culture enrich...

Embodiment 2

[0043] Microbial nitrite degradation agent and preparation method, comprising the following steps:

[0044]The first step: Bacillus subtilis is inoculated in liquid culture medium, and it is prepared by the raw material of following percentage by weight: bean cake powder 3%, corn flour 5%, molasses 1.0%, potassium dihydrogen phosphate 0.05%, disodium hydrogen phosphate 0.4%, silicone defoamer 0.06%, water 90.49%, the culture temperature is 37°C, and the culture time is 32 hours;

[0045] Second step: inoculate the nitrifying bacteria in the enrichment medium, which is prepared by the following raw materials in weight percentage: 0.7% of ammonium sulfate, 1.2% of sodium bicarbonate, 0.6% of sodium chloride, 0.06% of magnesium sulfate heptahydrate, seven Ferrous sulfate water 0.03%, calcium chloride dihydrate 0.1%, glucose 0.1%, water 97.21%; control pH 8.0~8.5, temperature 28~30℃, Vs: 30%~40%, (DO) > 2mg L -1, carry out continuous culture enrichment, maintain the control param...

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PUM

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Abstract

The invention relates to a biological nitrite-degrading agent. It is characterized in that it comprises following steps: culturing bacillus subtilis and nitration bacteria separately, solidifying culturing product with centrifugal, absorption and alginate-calcium chloride embedding, mixing and granulating to get said product. The invention is characterized in that it employs special fermentation method to make product posses a large quantity of bacteria and active physiological metabolism; it makes use of composite solidifying method to make various elements be fixed on special carrier for effective and stable wastewater treatment.

Description

technical field [0001] The invention relates to a microbial nitrite degradation agent and a production method thereof, which can be used in water treatment projects of sewage treatment, aquaculture, aquariums or flowing water systems, and belong to the technical field of sewage treatment by microorganisms. Background technique [0002] Nitrite is an intermediate product in the process of converting ammonia into nitrate. In the aquaculture water, the residual bait left by a large amount of bait, the accumulation of a large amount of excrement of aquatic animals in the water, and the regular use of disinfectants will cause harmful The harmful and beneficial bacteria are all killed, and the supply of oxygen is insufficient, resulting in the obstruction of the nitrification process of a large amount of accumulated nitrogen, resulting in high content of ammonia nitrogen and nitrite nitrogen in the water during breeding, but due to the rapid conversion of ammonia nitrogen, the nitr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125
Inventor 江瀚马正驰
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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