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Immunoassay product and process

An immunoassay, flow distributor technology, applied in the field of positional devices, can solve problems such as uncontrollability

Active Publication Date: 2007-06-13
MILLIPORE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they were skeptical about the work as it is only suitable for small volume applications and is still not controllable

Method used

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  • Immunoassay product and process
  • Immunoassay product and process
  • Immunoassay product and process

Examples

Experimental program
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Embodiment Construction

[0050] As shown in Figure 1, the holder 2 consists of two parts. The first or lower part is the porous support 4 . Preferably, the support has a rim 6 or mounting means designed to be mounted in or on the manifold 8 (FIG. 3). One or more imprinted membranes (not shown) are placed on the support 4 such that the bottom surface of the one or more membranes is in contact with the upper surface of the support. The second part of the holder 2 is a porous flow distributor (not shown) immediately above the one or more imprinted membranes.

[0051] The top 10 and bottom 4 components are preferably bonded to each other, at least during use, so as to securely hold the one or more membranes. These two parts 4 and 10 of the holder 2 are connected together by a hinge 16 as shown in FIGS. 1 and 2 . As shown in this example, the "hinge" is a "living" hinge connecting the two parts together. Alternatively, the hinges can be fabricated separately and attached using adhesives, thermal adhesi...

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Abstract

The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Provisional Application No. 60 / 795,452, filed April 27, 2006, U.S. Provisional Application No. 60 / 795,532, filed April 27, 2006, and U.S. Provisional Application No. 60 / 795,532, filed November 3, 2005. Priority of Provisional Application No. 60 / 732994. technical field [0003] The present invention relates to a device and method for detecting the position of substances contained in blotted membranes. More particularly, it relates to a technique for rapid detection by vacuum or positive pressure by applying reagents and washing solutions to a blotted membrane. Background technique [0004] The use of gel electrophoresis is currently a common technique for separating biological materials. (Non-biological materials can also be separated using gels or other chromatographic supports, but more work has been done on biological materials). Typical applications include isolating nucleic acid frag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 马渊雅治木村广子马克·埃梅里克菲利普·克拉克库尔特·格里尼兹恩
Owner MILLIPORE CORP
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