Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing heart fatty acid binding protein monoclonal antibody and its use

A combination technology of monoclonal antibody and fatty acid, applied in the direction of anti-animal/human immunoglobulin, measuring devices, instruments, etc., to achieve high-precision results

Inactive Publication Date: 2007-06-13
上海单抗制药技术有限公司
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it can be treated within 4-6 hours of the onset, the patient has a great chance of recovery. If it passes 6 hours, it is often too late to rescue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing heart fatty acid binding protein monoclonal antibody and its use
  • Method for preparing heart fatty acid binding protein monoclonal antibody and its use
  • Method for preparing heart fatty acid binding protein monoclonal antibody and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Preparation of monoclonal antibodies by hybridoma cell method:

[0083] A. Antibody Preparation

[0084] 1. The BalB / C mice were immunized by a conventional method, and the immunogen was natural H-FABP protein isolated and purified from human heart tissue (purchased from Life Diagnostic Company of the United States);

[0085] 2. For the first immunization, fully emulsify 100 micrograms of the antigen with Freund's complete adjuvant, and then inject it intraperitoneally or subcutaneously at multiple points. After that, every two weeks, inject 50 micrograms of antigen mixed with incomplete adjuvant, and immunize four times in total;

[0086] 3. Immunization was boosted once 3 days before fusion, and then the spleen was taken out to prepare a spleen cell suspension;

[0087] 4. fused with SP2 / 0 cells under the action of PEG fusion agent, and screened single clones on HAT selection medium;

[0088] 5. After several fusions, 15 anti-H-FABP specific monoclonal antibody str...

Embodiment 2

[0106] Preparation of monoclonal antibody by mouse ascites method:

[0107] A. Antibody preparation:

[0108] 1. The mice used to prepare ascites are all SPF grade mice. The production mice have been inspected and have qualified certificates. If the animals are unhealthy, bitten or infected during the production process, they will be discarded immediately.

[0109] 2. Animal equipment and facilities: All animal experiments were carried out in the Animal Center of Shanghai Second Medical University, which is an SPF animal room and complies with national regulations.

[0110] 3. Establishment of cell bank: In order to ensure the long-term and stable production of high-quality monoclonal antibodies that meet the requirements, a high-standard cell bank must first be established, including the original cell bank, seed cell bank and production cell bank, the location and generation of cell tubes Special personnel are responsible for the number of tubes, cryopreservation, and resusc...

Embodiment 3

[0121] Antibody Sequencing

[0122] The currently applied rapid sequencing technique is the enzymatic method proposed by Sanger et al. (1977). Firstly, several groups of fluorescently labeled oligonucleotides are synthesized independently of each other. Each group of oligonucleotides has a fixed starting point, but ends randomly at one or more specific residues. Since each base on the DNA has an equal chance of appearing at the variable termination end, each of the above-mentioned products is a mixture of oligonucleotides whose length is determined by a specific base in the original DNA. Determined by the position on the fragment. Then, under conditions that can distinguish different DNA molecules with a length difference of only one nucleotide, electrophoresis analysis is performed on each group of oligonucleotides, as long as several groups of oligonucleotides are added to several adjacent DNA molecules in the sequencing gel. On the swimming lane, the nucleotide sequence o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The purpose of this invention is to provide monoclonal antibodies which specific binding with heart-type fatty acid-binding protein (H-FABP), especially a pair of monoclonal antibodies specific binding with the heart-type fatty acid-binding protein (H-FABP), can develop specific, high accuracy kit in the early diagnosis of acute myocardial infarction.

Description

technical field [0001] The invention relates to an antibody specifically binding to heart-type fatty acid binding protein (H-FABP), especially a monoclonal antibody specifically binding to heart-type fatty acid binding protein (H-FABP) and a preparation method thereof. Background technique [0002] Cardiovascular diseases have always been the enemy of people's health. In recent years, with the improvement of living standards and the increasing social pressure of work, the incidence of cardiovascular disease has increased over the years, and heart disease has become the second leading cause of death after cancer. In heart disease, especially ischemic heart disease such as angina pectoris and myocardial infarction, the increase is particularly significant, accounting for about 40% of the number of deaths due to heart disease. [0003] Fatty acid binding protein (H-FABP) is a group of proteins (14,000-15,000) composed of at least 6 different relative molecular masses. In diff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/18C12N15/09G01N33/577
Inventor 陈克勤劳烨黄大庆包维丽朱亚郑健红
Owner 上海单抗制药技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com