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Pentachlorophenol polluted soil united directional restoration method using 'plant-microorganism'

A technology of polluted soil and joint orientation, which is applied in the restoration of polluted soil and other directions, can solve the problems of difficult survival, inability to repair PCP polluted soil, and incompatibility.

Inactive Publication Date: 2007-05-30
TIANJIN ACADEMY OF ENVIRONMENTAL SCI
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Problems solved by technology

[0003] Different from the above-mentioned research results, because the soil environment in the present invention is located in the coastal area, its salt content is very high, it is difficult for simple PCP degrading bacteria to survive, or even if it survives, it will be difficult to play a role because it is not suitable for the environment, thus Cannot play the role of repairing PCP polluted soil

Method used

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Examples

Experimental program
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Embodiment Construction

[0032] The present invention is described in further detail below by way of examples. It should be understood that the described examples are only for illustration and not limitation of the invention.

[0033] 1. Isolation of PCP degrading bacteria Psendomonas sp. and Bacillus sp.

[0034] Select appropriate mud samples and inoculate them into PCP-enriched medium for culture, with an initial PCP concentration of 50 mg / l, and culture them on a shaker at 30°C and 180 rpm for 24 hours. The content of NaCl in the basic inorganic salt medium used in this experiment is 1%, which is set for the purpose of screening salt-tolerant PCP degrading bacteria to adapt to the high salt content of coastal soil. After the culture medium is turbid, the activated sludge stock solution is obtained. The activated sludge stock solution was transferred to 500ml enriched medium for acclimatization. The time for the sludge to degrade PCP was greatly shortened when the sludge was repeatedly acclimate...

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Abstract

A plant-microbe joint directional restoring penchlorol contaminated soil starts from selecting specific high degrading PCP degrading bacteria, then testing them and choosing optimum cultivating conditions. With soils of different degree of contamination, it can be used for potting plant suaeda glauce testing soil. Test the PCP left quantity in different growing stage of the suaeda glauce through sampling of the soil. It has the highest PCP degrading rate, with low density PCP (5mg / kg) reaching 94.73%. Combining of the specific PCP degrading bacteria and planting saeda glauce, restoring effect of soil is far bigger than implementing either one of them, reaching optimum PCP degrading result.

Description

technical field [0001] The invention belongs to a method for transforming damaged coastal soil, in particular to a method for jointly and directionally repairing pentachlorophenol-contaminated soil by "plants and microorganisms". Background technique [0002] Most of Tianjin’s coastal areas along the Bohai Sea are tidal flats and wetlands with high salinity. In recent years, due to the intensification of industrial pollution, the pollution of the soil along the Bohai Sea by the South Drainage River, which is mainly fed by urban domestic sewage and industrial sewage, has also become increasingly serious. Coupled with the unreasonable use of a large amount of pesticides in the farmland in the surrounding areas, the concentration and types of various highly toxic organic substances contained in the soil have also greatly increased. Among them, the accumulation of chlorophenol compounds, especially pentachlorophenol (abbreviated as PCP) in the soil has become the most important ...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
Inventor 李小宁郑先强孙贻超孙静张征云李红柳邵晓龙侯晓珉
Owner TIANJIN ACADEMY OF ENVIRONMENTAL SCI
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