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Extraction of microbiological polysaccharide-hot gel

A technology of microbial polysaccharide and extraction process, applied in bacteria, fermentation and other directions, can solve problems such as impracticality, and achieve the effects of good performance, cost reduction and ethanol consumption saving

Inactive Publication Date: 2007-04-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in industrial production, these methods are not practical

Method used

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  • Extraction of microbiological polysaccharide-hot gel
  • Extraction of microbiological polysaccharide-hot gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1. Preparation of thermogel fermented liquid

[0012] Strain Alcaligenes faecalis (Alcaligenes faecalis) WX-C12 was preserved and provided by the Biochemical Engineering Laboratory of the School of Bioengineering, Jiangnan University.

[0013] Fermentation medium (g / L) glucose 50, yeast extract 2, NH 4 Cl 3.6, KH 2 PO 4 2. MgSO 4 ·7H 2 O 0.5, inorganic salt concentrated solution 10mL, pH 7.0 (wherein inorganic salt concentrated solution: FeCl 3 1g, NaCl 1g, CaCl 2 1g, MnCl 2 1 g, 1 L deionized water).

[0014] Utilize 9L fermentation medium, adopt two-stage method (different pH values ​​are controlled during bacterial cell growth and rubber production stage) to carry out fermentation on 15L Biostat C10-3 mechanical stirring tank, the inoculum size is 5%, the stirring speed of the fermenter is 500rpm, and the ventilation volume is 13.5L / min, gauge pressure 0.05MPa, fermentation temperature 30°C. The concentration of NaOH solution to adjust the pH v...

Embodiment 2

[0016] Embodiment 2. Preparation of thermogel fermented liquid

[0017] Bacterial strain and fermentation medium are with embodiment 1. Utilize 18L fermentation medium, inoculum amount 5%, adopt two-stage method (thalline growth and gel production stage control different pH value) to carry out fermentation on the 25L mechanical stirring tank, the stirring speed of fermentation tank is 300rpm, and ventilation rate is 27L / min, The gauge pressure is 0.05MPa, and the fermentation temperature is 30°C. The pH value is controlled at about 7.0 during the growth stage of the bacteria, and adjusted to about 5.6 after 21 hours until the end of fermentation.

[0018] The initial glucose concentration of the fermentation was 50g / L, and the glucose concentration in the fermentation broth dropped below 10g / L after 43 hours of fermentation, and 720g of glucose was added, and the glucose concentration in the fermentation broth dropped to below 10g / L again at 79h, and 720g of glucose was added...

Embodiment 3

[0019] Example 3. Extraction of crude product thermal gel

[0020] Take 2L of fermentation broth, centrifuge at 5000rpm for 15min, and centrifuge and wash the precipitate four times with deionized water at 4°C, dehydrate by centrifugation, then wash with ethanol, dehydrate by centrifuge, dry in vacuum at 37°C, and pulverize to obtain a crude thermogel. Product properties are shown in Table 1:

[0021] fermentation broth

[0022] The extraction process is not dissolved by alkali, and the triple helix conformation of the thermal gel molecule is not destroyed, so the thermal gelation performance is good, but the bacteria are not removed, and the purity is slightly low.

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Abstract

Extraction of microbial polysaccharide-thermal gel is carried out by taking fecal alkali bacterium as fermented broth, centrifugalizing to obtain deposit, washing for 4 times by deionized water, washing by alcohol, drying and crushing to obtain the final product. It costs low.

Description

[0001] This application is a divisional application with the application date of June 11, 2004, the application number is 200410041271.8, and the title of the invention is the same as above technical field [0002] The invention discloses a microbial polysaccharide-thermogel extraction process, which belongs to the technical field of bioengineering. Background technique [0003] Thermogel, also known as coagulation polysaccharide, thermocoagulation polysaccharide, English name Curdlan, was discovered and studied by T. Harada of Osaka University in Japan in 1964 when screening microorganisms that can metabolize various petroleum components. Thermogel is a new type of microbial exopolysaccharide produced by fermentation of Alcaligenes faecalis var. myxogene with carbohydrates as the main raw material. Its molecular formula is (C 6 h 10 o 5 ) n , Intramolecularly connected by glucose structural units with β-1,3 bonds. Its chemical structure is shown below: [0004] [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12N1/20C12P19/04
Inventor 詹晓北朱莉
Owner JIANGNAN UNIV
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