Observing device and fluorescent light observing device

Abstract

An image can be captured with a high resolution to improve an observing accuracy without excessively reducing a numeric aperture despite a smaller magnification. A microscope observing device comprising a light source for applying an exciting light or a lighting light to a specimen placed on a stage, an object lens disposed oppositely on the stage to magnify a fluorescent light or a reflection light from the specimen, an imaging lens for forming an image on the specimen magnified by the object lens, and an imaging means for picking up the image on the specimen formed by the imaging lens, wherein a mechanism for switching object lenses is provided along with a plurality of object lenses with different magnifications, and a mechanism for switching imaging lenses (5a, 5b) is provided along with a plurality of imaging lenses with different magnifications.

Description

technical field [0001] The present invention relates to an observation device such as a microscope observation device and a fluorescence observation device for observing living bodies, organs, tissues, and the like. Background technique [0002] Conventionally, a microscope observation device disclosed in Patent Document 1 has been known as a microscope observation device. The microscope observation device has: an objective lens arranged opposite to the sample; an imaging lens for imaging a magnified image in an imaging unit such as a CCD camera; A zoom relay lens that continuously changes the magnification inside. [0003] According to this microscope observation device, the objective lens and the imaging lens are fixed, and even if the telephoto zoom relay lens is inserted and removed between them, the parfocal position of the imaging surface does not change, and the deterioration of the image due to zooming is small, and the Improve operability or performance. [0004]...

AI Technical Summary

Problems solved by technology

[0006] However, since conventional microscope observation devices change the observation magnification by changing the magnification of the telephoto zoom relay lens, it is difficult to change the magnification within a wide range of magnifications.

That is, since the same objective lens and imaging lens are used from a smaller magnification to a larger magnification, there is an inconvenience that the numerical aperture is too small and the resolution decreases at a smaller magnification

[0007] In addition, in the conventional observation device, when the observed sample is a living body, an organ or a tissue, since the thicker they are, the more easily the illuminating light is absorbed, so it is difficult to effectively detect the target in the living body, organ or tissue. Illumination of the observed area

[0008] In addition, since the thicker the organism, organ or tissue is, the more fluorescence it has, so it is difficult to observe the part to be observed in the organism, organ or tissue with better resolution

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