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Gamma-polyglutamic acid generating bacterium and process of preparing gamma-polyglutamic acid therewith

A polyglutamic acid and bacteria-producing technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as high material prices and achieve the effect of reducing raw material costs

Active Publication Date: 2007-03-21
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, L-glutamic acid as a raw material has disadvantages such as expensive materials

Method used

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  • Gamma-polyglutamic acid generating bacterium and process of preparing gamma-polyglutamic acid therewith

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Effect test

Embodiment 1

[0028] Take slant seeds stored in a refrigerator at 4°C for 1 day [composition of preservation slant medium (g / L): peptone 10, NaCl 10, yeast extract 5, agar 20, pH 7.0. ], use an inoculation needle to place about 1cm on the above slope 2 The strains of the area were scraped off and transferred to the newly configured fermentation medium. The composition (g / L) of the fermentation medium was as follows: 80g fructose, 18gNH4 Cl, 1.5g K 2 HPO 4 , 0.05gMgSO 4 ·7H 2 O, 0.01g FeCl 3 ·6H 2 O, 0.15g CaCl 2 ·2H 2 O, 0.05g MnSO 4 ·H 2 O, and adjusted the pH of the medium (before sterilization) to 6.5 with NaOH and HCl, and sterilized at 120° C. for 20 minutes. The fermentation medium was packed in a 500ml conical flask, with a filling volume of 20ml, and was shaken and cultivated on a reciprocating shaker with a stroke of 76mm and a rotation speed of 130r / m. After centrifugation for 10 minutes, the content of γ-PGA was determined by paper chromatography. Using this method, th...

Embodiment 2

[0030] Take slant seeds stored in a refrigerator at 4°C for 2 days [composition of preservation slant medium (g / L): peptone 8, NaCl 11, yeast extract 6, agar 20, pH 7.0. ], use an inoculation needle to place about 1cm on the above slope 2 The strains in the area were scraped off and transferred to the newly configured fermentation medium. The composition (g / L) of the fermentation medium was as follows: 90g sucrose, 20g (NH4) 4 ) 2 SO 4 , 1.0g K 2 HPO 4 , 0.06g MgSO 4 ·7H 2 O, 0.01g FeCl 3 ·6H 2 O, 0.15g CaCl 2 ·2H 2 O, 0.05g MnSO 4 ·H 2 O, and adjusted the pH of the medium (before sterilization) to 6.5 with NaOH and HCl, and sterilized at 120° C. for 20 minutes. The fermentation medium was placed in a 500ml conical flask, with a volume of 20ml, and was shaken and cultured on a reciprocating shaker with a stroke of 76mm and a rotational speed of 120r / m. After centrifugation for 10 minutes, the content of γ-PGA was determined by paper chromatography. The γ-PGA prod...

Embodiment 3

[0032] Take slant seeds stored in a refrigerator at 4°C for 3 days [composition of preservation slant medium (g / L): peptone 12, NaCl 9, yeast extract 4, agar 20, pH 7.0. ], use an inoculation needle to place about 1cm on the above slope 2 The strains of the area were scraped off and transferred to the newly configured fermentation medium. The composition (g / L) of the fermentation medium was as follows: 60g glucose, 20g NH 4 NO 3 , 1.0g K 2 HPO 4 , 0.01gMgSO 4 ·7H 2 O, 0.05g FeCl 3 ·6H 2 O, 0.2g CaCl 2 ·2H 2 O, 0.104g MnSO 4 ·H 2 O, and adjusted the pH of the medium (before sterilization) to 7.0 with NaOH and HCl, and sterilized at 120° C. for 20 minutes. The fermentation medium was packed in a 500ml conical flask, with a filling volume of 20ml, and was shaken and cultured on a reciprocating shaker with a stroke of 65mm and a rotation speed of 90r / m. After centrifugation for 10 minutes, the content of γ-PGA was determined by paper chromatography. Using this method,...

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Abstract

The present invention provides one kind of gamma-polyglutamic acid generating bacterium and the process of preparing gamma-polyglutamic acid (gamma-PGA) therewith. The gamma-polyglutamic acid generating bacterium is Bacillus substilis CCTCC M 206102. The preparation process of gamma-PGA includes using Bacillus substilis CCTCC M 206102 as fermenting strain, and shake flask fermentation of sugar material without adding L-glutamic acid. The present invention has relatively low material cost, gamma-PGA output up to 18g / L and excellent industrial application foreground.

Description

technical field [0001] The present invention relates to a microorganism strain for producing γ-polyglutamic acid and a method for fermenting and producing γ-polyglutamic acid by utilizing the strain. Background technique [0002] In 1937, Ivanovics et al. first discovered that the capsule of Bacillus anthracis contains γ-polyglutamic acid (γ-PGA). In 1942, Bovarnick et al. found that some Bacillus bacteria can accumulate γ-PGA through fermentation culture. In 1973, Troy discovered that γ-polyglutamic acid (hereinafter referred to as γ-PGA) is a chemical component of Bacillus anthracis cell membrane, which is a water-soluble amide compound that can be produced by a variant of Bacillus. Its structure is unique. It is a multimer connected by an amide bond formed by a single glutamic acid molecule at γ-hydroxyl and α-amino group. Its structural formula is as follows: [0003] [0004] γ-PGA is made from L-glutamic acid (some strains may not need to add L-glutamic acid) throu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/14C12R1/125
Inventor 施庆珊李诚斌欧阳友生陈仪本疏秀林谢小保黄小茉
Owner GUANGDONG INST OF MICROORGANISM
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