Fast and high performance method for filtrating out halophilic bacteria

A technology for halophilic bacteria and moderately halophilic bacteria, applied in the direction of bacteria, etc., can solve the problems of unfavorable bacterial growth, slow growth of halophilic bacteria, increased osmotic pressure, etc. improved effect

Inactive Publication Date: 2007-02-21
HARBIN INST OF TECH
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the past, people used the plate separation method to screen halophilic bacteria. However, due to the slow growth of halophilic bacteria on the plate, and the excessive evaporation of water on the plate during long-term culture, the salinity around the bacteria increased greatly, and the osmotic pressure increased. It is beneficial to the growth o

Method used

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specific Embodiment approach 1

[0010] Specific embodiment one: this embodiment screens halophilic bacteria strains according to the following method:

[0011] (1) Sampling: Halophilic bacteria mainly grow in salt lakes, saline lakes, salt marshes, dead seas, dead valleys, salt farms, salt ponds, saline lands, oceans, deserts, salted objects (such as pickled fish, pickled vegetables, salted animal skins) and other salt products) and enterprises (such as leather, paper, cheese, meat, seafood processing, oil extraction and processing, food canning, oil fermentation, dyes, pesticides, pharmaceutical intermediates, neoprene, petroleum and Natural gas recovery, printing and dyeing, seawater utilization in coastal areas seeps into urban sewers, etc.) High-salt wastewater discharged into high-salt environments. Select sampling sites according to the purpose of halophilic bacteria research, and then collect water samples, mud samples or soil samples according to aseptic procedures.

[0012] (2) The formula of the m...

specific Embodiment approach 2

[0014] Specific embodiment two: According to the method for fast and efficient screening of halophilic bacteria of the present invention, 68 strains of bacteria were isolated from the high-salt environment of Weihai City in only 16 days, and the morphological characteristics, cultural characteristics, physiological and biochemical and nucleic acid analysis of these bacterial strains were carried out. Characteristic identification, 100% of the identified strains were halophilic strains. The traditional method usually takes 40-70 days to isolate and purify a strain of halophilic bacteria (different strains are different), and this method takes 12-20 days, increasing the efficiency by 3-4 times. Specific steps are as follows:

[0015] (1) Sample source

[0016] The samples were collected from: brine and salt mud from sun-dried salt ponds at Ludaokou Salt Field in Weihai City; high-salt wastewater and sludge discharged by Weihai Jinhong Chemical Co., Ltd.; seawater and sea mud fr...

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Abstract

This invention relates to a test tube slant culture method for rapidly and effectively screening Vibrio parahaemolyticus. The method comprises: (1) placing the collected Vibrio parahaemolyticus sample into a Fernbach culture flask containing liquid culture medium, and culturing by shaking in light; (2) collecting enrichment culture liquid by an inoculating loop, and laying a curve onto the prepared test tube culture medium slant; (3) culturing in a culture case to obtain individual slant colonies; (4) observing the slant, selecting a conoly characteristic of Vibrio parahaemolyticus, laying a curve onto the prepared test tube culture medium slant, culturing, repeating for 3 times, separating and purifying to obtain cultured Vibrio parahaemolyticus strain. The method adopts test tube slant culture method, and can realize rapid and effective screening of Vibrio parahaemolyticus. The efficiency can be raised by 3-4 times.

Description

technical field [0001] The invention relates to a method for quickly and efficiently screening halophilic bacteria by using a test tube inclined plane culture method. Background technique [0002] In recent years, a variety of new life forms have been discovered in many areas that were previously considered to be forbidden areas for life. The places where they survive and reproduce are precisely some extreme environments, such as halophilic bacteria, thermophilic bacteria, psychrophilic bacteria, acidophilic bacteria, alkaliphilic bacteria, barophilic bacteria, etc., which are collectively referred to as extremophiles. Extremophiles have unique gene types, special physiological mechanisms and special metabolites. As marginal life phenomena on the earth, extremophiles are quite intriguing. It will give people many important enlightenments on the origin of life, system evolution, etc., and will expand people's concepts on the principles of life behavior. At present, extremop...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 马放李维国苏俊峰王光玉
Owner HARBIN INST OF TECH
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