Fast and high performance method for filtrating out halophilic bacteria

Abstract

This invention relates to a test tube slant culture method for rapidly and effectively screening Vibrio parahaemolyticus. The method comprises: (1) placing the collected Vibrio parahaemolyticus sample into a Fernbach culture flask containing liquid culture medium, and culturing by shaking in light; (2) collecting enrichment culture liquid by an inoculating loop, and laying a curve onto the prepared test tube culture medium slant; (3) culturing in a culture case to obtain individual slant colonies; (4) observing the slant, selecting a conoly characteristic of Vibrio parahaemolyticus, laying a curve onto the prepared test tube culture medium slant, culturing, repeating for 3 times, separating and purifying to obtain cultured Vibrio parahaemolyticus strain. The method adopts test tube slant culture method, and can realize rapid and effective screening of Vibrio parahaemolyticus. The efficiency can be raised by 3-4 times.

Description

technical field [0001] The invention relates to a method for quickly and efficiently screening halophilic bacteria by using a test tube inclined plane culture method. Background technique [0002] In recent years, a variety of new life forms have been discovered in many areas that were previously considered to be forbidden areas for life. The places where they survive and reproduce are precisely some extreme environments, such as halophilic bacteria, thermophilic bacteria, psychrophilic bacteria, acidophilic bacteria, alkaliphilic bacteria, barophilic bacteria, etc., which are collectively referred to as extremophiles. Extremophiles have unique gene types, special physiological mechanisms and special metabolites. As marginal life phenomena on the earth, extremophiles are quite intriguing. It will give people many important enlightenments on the origin of life, system evolution, etc., and will expand people's concepts on the principles of life behavior. At present, extremop...

AI Technical Summary

Problems solved by technology

[0005] In the past, people used the plate separation method to screen halophilic bacteria. However, due to the slow growth of halophilic bacteria on the plate, and the excessive evaporation of water on the plate during long-term culture, the salinity around the bacteria increased greatly, and the osmotic pressure increased. It is beneficial to the growth of bacteria, and then makes the growth of halophilic bacteria more slowly, which makes it difficult to screen halophilic bacteria. Often, purified halophilic bacteria strains cannot be obtained for a long time, and the efficiency of strain screening is extremely low, making halophilic bacteria this valuable of biological resources cannot be rapidly developed