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Determination method of pesticide bioactivity

A technology of biological activity and determination method, applied in the field of pesticide bioassay, can solve the problems of missed screening of active compounds, sensitive to pesticide activity, unable to screen out compounds with insecticidal activity, etc., achieving good indication and representativeness, and easy to raise in large quantities Effect

Inactive Publication Date: 2007-01-31
上海南方农药研究中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tests have shown that daphnia and larvae are only sensitive to some pesticide activities, and other potential compounds with insecticidal activity cannot be screened out.
At present, the success rate of new pesticide development in the world is getting lower and lower, so the requirements for screening are higher
However, the current screening method inevitably misses the active compound.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~6

[0021] According to the above test method, the insecticidal activity of trichlorfon technical, methethrin technical, imidacloprid technical, depletion technical, flubendron technical and m-trifluoromethyl urea were measured respectively. The contents and conditions are shown in Table 1, and the results of the measurement are shown in Table 2.

[0022] Example

Determination of samples

solvent

Emulsifier

Measuring vessel

Drug concentration (mg / L)

1

Trichlorfon technical

acetone

Tween 80

5ml small beaker

0.1

1

10

50

100

2

The original drug of pyrethrin

acetone

Tween 80

10ml small beaker

0.1

1

10

50

100

3

Imidacloprid technical

acetone

Tween 80

48-well plate

0.1

1

10

50

100

4

Eliminate the original drug

Acetone+DMF

Tween 80

96-well plates

0.1

1

...

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PUM

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Abstract

The invention relates to a pesticide biological activity testing method that uses Cyclops as acting object. In slight amount vessel, the sensitivity of the sample would be used to take insecticidal activity test by Cyclops. The method is easy to operate and is suited to test biology of slight amount sample. The method could be used to test the activity of the existing variety and research the new pesticides.

Description

technical field [0001] The invention belongs to the field of pesticide bioassay, and in particular relates to a pesticide bioactivity assay method using Crustacea as a test object. Background technique [0002] An important part in the research and development of new pesticides is the biological screening of new pesticides. With the deepening of research cognition and the continuous improvement of technical means, the inventory of test compounds is becoming more and more large, the source is diversified and the test samples are gradually microquantified. The test compound samples are not limited to chemical samples artificially synthesized in the laboratory. A large number of combined chemical samples, machine-automatically synthesized chemical samples, combined biochemical samples, microbial-derived compounds, and plant-derived compounds have also been added. The amount of these samples or synthetic samples is very small, or the active ingredient content is very low. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 张雅凤
Owner 上海南方农药研究中心
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