Reagent determination by serum potassium ion enzyme method

Abstract

The present invention discloses the enzymatic measurement reagents and process of potassium ion in serum. Crown ether is used in the present invention that has the characteristics of cryptand ether and is capable of complexing with potassium ion. Different matches of reagents are tested to find out the proper concentration. The present invention has similar result with the cryptand ether method in the aspects of sensitivity, accuracy and precision, but it is much simpler and decreases the cost significantly.

Description

Serum Potassium Ion Enzymatic Determination Reagent technical field The invention belongs to a clinical biochemical analysis method and is used for the determination of potassium ions in serum. Background technique In biochemical clinical experiments, the determination of serum potassium and sodium is a routine analysis item in hospitals. The normal value of potassium is 3.5-5.1mmol / L, and the common electrolyte disorder in clinical practice is hyperkalemia. The earliest method for the analysis of potassium ions was flame photometry. The principle of the method is that certain potassium atoms to be tested are excited by heating stimulation and emit light with a characteristic wavelength when returning to the ground state. The characteristic wavelength produced by the excited potassium atoms to be measured in the flame, the intensity of the radiant energy is directly proportional to the number of the excited potassium atoms to be measured in the flame, and the number of ...

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Problems solved by technology

The original electrode method not only needs to purchase additional instruments, but also needs to replace the electrodes frequently, and the consumption of electrolyte is relatively fast. These reasons make the cost of the electrode method to determine potassium ions in serum relatively high, while the enzymatic method does not have these problems , so the measurement cost is much lower

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