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Production for skin beautification and care, and preparation method

A technology for beauty and skin care products, which is applied in the field of beauty and skin care products. Its preparation field can solve the problems of single growth factor, lack of chemical modification of growth factors, lack of physiological compatibility of multiple growth factors, etc., to reduce allergens, and the method is simple and easy , good application prospects and operability effects

Active Publication Date: 2006-12-06
广州源生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In fact, various human growth factors such as bFGF and EGF have been widely used to manufacture beauty products, but there are two natural defects: 1) often a single growth factor is added, lacking the physiological compatibility of multiple growth factors
2) Since most of the currently used growth factors are derived from genetic engineering, the obtained growth factors often lack appropriate chemical modifications such as sugar modification

Method used

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  • Production for skin beautification and care, and preparation method
  • Production for skin beautification and care, and preparation method
  • Production for skin beautification and care, and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example provides the preparation process of the active ingredient derived from bone marrow mesenchymal stem cells. details as follows

[0048] 1. Culture and expansion of bone marrow-derived mesenchymal stem cells:

[0049] 1) Primary culture program: take bone marrow from healthy people, record blood type, take 5ml of bone marrow, add 5ml of PBS to dilute 1 times, beat and mix with 5ml syringe, take 15ml centrifuge tube, add 3ml of lymphocyte separation solution, change needle of 1ml syringe slowly Add diluted bone marrow fluid, pay attention to make the bone marrow fluid and lymphocyte separation fluid form a clear liquid surface; centrifuge at 2000rpm for 20 minutes; after centrifugation, obvious layers can be seen, and the middle white cloudy layer is the lymphocyte layer. Aspirate slowly, add 5ml of PBS, and centrifuge at 1000rpm for 5 minutes; discard the supernatant after centrifugation, add 10ml of PBS, mix with a pipette, and repeat centrifugation once; d...

Embodiment 2

[0060] This embodiment provides the preparation process of the active ingredient of umbilical cord blood stem cells, specifically as follows:

[0061] Cultivation and expansion of umbilical cord blood stem cells: take 5ml of umbilical cord blood, blood type test, record, add 5ml of PBS to dilute 1 times, beat and mix with a 5ml syringe, take a 15ml centrifuge tube, add 3ml of lymphocyte separation solution, change the needle of a 1ml syringe slowly Add diluted bone marrow fluid, pay attention to make the bone marrow fluid and lymphocyte separation fluid form a clear liquid surface; centrifuge at 2000rpm for 20 minutes; after centrifugation, obvious layers can be seen, and the white cloudy layer in the middle is the lymphocyte layer, suck it out slowly , add 5ml of PBS, and centrifuge at 1000rpm for 5 minutes; discard the supernatant after centrifugation, add 10ml of PBS, mix with a pipette, and repeat the centrifugation once; discard the supernatant, add 6ml of α-MEM medium con...

Embodiment 3

[0066] This embodiment provides the preparation process of the active ingredient of hematopoietic stem cells, specifically as follows:

[0067] Cultivation and expansion of hematopoietic stem cells: record blood type, take 5ml of blood, add 5ml of PBS to dilute 1 times, beat and mix with a 5ml syringe, take a 15ml centrifuge tube, add 3ml of lymphocyte separation medium, change the needle of a 1ml syringe and slowly add diluted bone marrow Take care to make the blood and lymphocyte separation liquid form a clear liquid surface; centrifuge at 2000rpm for 20 minutes; after centrifugation, obvious layers can be seen, and the white cloudy layer in the middle is the lymphocyte layer. Slowly suck it out, add 5ml PBS, Centrifuge at 1000rpm for 5 minutes; discard the supernatant after centrifugation, add 10ml of PBS, mix with a pipette, and repeat the centrifugation once; add CD34 antibody, stain at 4 degrees for 30 minutes, and sort by flow cytometry. CD34 positive cells are hematopoi...

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Abstract

A face-beautifying and skin-protecting product contains the a single blood type human stem cell as active component (0.10-50%) and is suitable for the people with same blood type. Its preparing process is also disclosed.

Description

technical field [0001] The invention relates to a cosmetic and skin care product and a preparation method thereof. The cosmetic and skin care product contains stem cell combination factors classified according to blood types, and belongs to the technical field of medical cosmetology. Background technique [0002] Stem cells are seed cells that have not yet differentiated and developed. They can self-replicate and differentiate into a variety of cells. These two aspects are the basis for stem cells to be used in skin care, beauty and even tissue regeneration. Stem cells are divided into embryonic stem cells and tissue-specific (adult) stem cells. Embryonic stem cells are derived from blastocysts developed after in vitro fertilization, while adult stem cells are derived from corresponding tissues, such as hematopoietic stem cells from bone marrow. In addition to the biological characteristics of stem cells, the self-replication and pluripotent differentiation of stem cells al...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61Q19/00A61Q19/10A61Q1/12
Inventor 李凌松马艳玲
Owner 广州源生医药科技有限公司
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